An electrochemical adaptation of Ellman's test

O Nekrassova, PC White, S Threlfell, G Hignett, AJ Wain, NS Lawrence, J Davis, RG Compton

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 muM, a limit of detection of 1.17 muM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.
    LanguageEnglish
    Pages797-802
    JournalANALYST
    Volume127
    Issue number6
    DOIs
    Publication statusPublished - Jun 2002

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    Sulfhydryl Compounds
    Cysteine
    Tissue
    Recovery

    Cite this

    Nekrassova, O., White, PC., Threlfell, S., Hignett, G., Wain, AJ., Lawrence, NS., ... Compton, RG. (2002). An electrochemical adaptation of Ellman's test. ANALYST, 127(6), 797-802. https://doi.org/10.1039/b202780c
    Nekrassova, O ; White, PC ; Threlfell, S ; Hignett, G ; Wain, AJ ; Lawrence, NS ; Davis, J ; Compton, RG. / An electrochemical adaptation of Ellman's test. In: ANALYST. 2002 ; Vol. 127, No. 6. pp. 797-802.
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    Nekrassova, O, White, PC, Threlfell, S, Hignett, G, Wain, AJ, Lawrence, NS, Davis, J & Compton, RG 2002, 'An electrochemical adaptation of Ellman's test', ANALYST, vol. 127, no. 6, pp. 797-802. https://doi.org/10.1039/b202780c

    An electrochemical adaptation of Ellman's test. / Nekrassova, O; White, PC; Threlfell, S; Hignett, G; Wain, AJ; Lawrence, NS; Davis, J; Compton, RG.

    In: ANALYST, Vol. 127, No. 6, 06.2002, p. 797-802.

    Research output: Contribution to journalArticle

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    AU - Lawrence, NS

    AU - Davis, J

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    AB - The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 muM, a limit of detection of 1.17 muM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.

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    Nekrassova O, White PC, Threlfell S, Hignett G, Wain AJ, Lawrence NS et al. An electrochemical adaptation of Ellman's test. ANALYST. 2002 Jun;127(6):797-802. https://doi.org/10.1039/b202780c