An assessment of endocrine activity in Australian rivers using chemical and in vitro analyses

Philip D. Scott, Michael Bartkow, Stephen J. Blockwell, Heather Coleman, Stuart J. Khan, Richard Lim, James A. McDonald, Helen Nice, Dayanthi Nugegoda, Vincent Pettigrove, Louis A. Tremblay, Michael St. J. Warne, Frederic D. L. Leusch

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Studies on endocrine disruption in Australia havemainly focused on wastewater effluents. Limited knowledgeexists regarding the relative contribution of different potentialsources of endocrine active compounds (EACs) to the aquaticenvironment (e.g., pesticide run-off, animal farming operations,urban stormwater, industrial inputs). In this study, 73river sites across mainland Australia were sampled quarterlyfor 1 year. Concentrations of 14 known EACs includingnatural and synthetic hormones and industrial compoundswere quantified by chemical analysis. EACs were detectedin 88 % of samples (250 of 285) with limits of quantification(LOQ) ranging from 0.05 to 20 ng/l. Bisphenol A (BPA; LOQ=20 ng/l) was the most frequently detected EAC (66 %) andits predicted no-effect concentration (PNEC) was exceeded 24times. The most common hormone was estrone, detected in28 % of samples (LOQ=1 ng/l), and the PNEC was alsoexceeded 24 times. 17α-Ethinylestradiol (LOQ=0.05 ng/l)was detected in 10 % of samples at concentrations rangingfrom 0.05 to 0.17 ng/l. It was detected in many sampleswith no wastewater influence, and the PNEC wasexceeded 13 times. In parallel to the chemical analysis,endocrine activity was assessed using a battery ofCALUX bioassays. Estrogenic activity was detected in19 % (53 of 285) of samples (LOQ=0.1 ng/l 17β-estradiol equivalent; EEQ). Seven samples exhibitedestrogenic activity (1–6.5 ng/l EEQ) greater than the PNECfor 17β-estradiol. Anti-progestagenic activity was detected in16 % of samples (LOQ=8 ng/l mifepristone equivalents;MifEQ), but the causative compounds are unknown. Withseveral compounds and endocrine activity exceeding PNECvalues, there is potential risk to the Australian freshwaterecosystems.
LanguageEnglish
Pages12951-12967
JournalEnvironmental Science and Pollution Research
Volume21
Issue number22
Early online date2 Jul 2014
DOIs
Publication statusE-pub ahead of print - 2 Jul 2014

Fingerprint

Hormones
Waste Water
Rivers
Estradiol
Wastewater
Mifepristone
chemical analysis
Ethinyl Estradiol
hormone
Bioassay
Estrone
Pesticides
Agriculture
Chemical analysis
river
Biological Assay
wastewater
Effluents
Animals
stormwater

Keywords

  • AR-CALUX . EDC . Endocrine disruption .
  • ER-CALUX . Ethinylestradiol . PR-CALUX

Cite this

Scott, Philip D. ; Bartkow, Michael ; Blockwell, Stephen J. ; Coleman, Heather ; Khan, Stuart J. ; Lim, Richard ; McDonald, James A. ; Nice, Helen ; Nugegoda, Dayanthi ; Pettigrove, Vincent ; Tremblay, Louis A. ; Warne, Michael St. J. ; Leusch, Frederic D. L. / An assessment of endocrine activity in Australian rivers using chemical and in vitro analyses. In: Environmental Science and Pollution Research. 2014 ; Vol. 21, No. 22. pp. 12951-12967.
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author = "Scott, {Philip D.} and Michael Bartkow and Blockwell, {Stephen J.} and Heather Coleman and Khan, {Stuart J.} and Richard Lim and McDonald, {James A.} and Helen Nice and Dayanthi Nugegoda and Vincent Pettigrove and Tremblay, {Louis A.} and Warne, {Michael St. J.} and Leusch, {Frederic D. L.}",
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Scott, PD, Bartkow, M, Blockwell, SJ, Coleman, H, Khan, SJ, Lim, R, McDonald, JA, Nice, H, Nugegoda, D, Pettigrove, V, Tremblay, LA, Warne, MSJ & Leusch, FDL 2014, 'An assessment of endocrine activity in Australian rivers using chemical and in vitro analyses', Environmental Science and Pollution Research, vol. 21, no. 22, pp. 12951-12967. https://doi.org/10.1007/s11356-014-3235-7

An assessment of endocrine activity in Australian rivers using chemical and in vitro analyses. / Scott, Philip D.; Bartkow, Michael; Blockwell, Stephen J.; Coleman, Heather; Khan, Stuart J.; Lim, Richard; McDonald, James A.; Nice, Helen; Nugegoda, Dayanthi; Pettigrove, Vincent; Tremblay, Louis A.; Warne, Michael St. J.; Leusch, Frederic D. L.

In: Environmental Science and Pollution Research, Vol. 21, No. 22, 02.07.2014, p. 12951-12967.

Research output: Contribution to journalArticle

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T1 - An assessment of endocrine activity in Australian rivers using chemical and in vitro analyses

AU - Scott, Philip D.

AU - Bartkow, Michael

AU - Blockwell, Stephen J.

AU - Coleman, Heather

AU - Khan, Stuart J.

AU - Lim, Richard

AU - McDonald, James A.

AU - Nice, Helen

AU - Nugegoda, Dayanthi

AU - Pettigrove, Vincent

AU - Tremblay, Louis A.

AU - Warne, Michael St. J.

AU - Leusch, Frederic D. L.

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AB - Studies on endocrine disruption in Australia havemainly focused on wastewater effluents. Limited knowledgeexists regarding the relative contribution of different potentialsources of endocrine active compounds (EACs) to the aquaticenvironment (e.g., pesticide run-off, animal farming operations,urban stormwater, industrial inputs). In this study, 73river sites across mainland Australia were sampled quarterlyfor 1 year. Concentrations of 14 known EACs includingnatural and synthetic hormones and industrial compoundswere quantified by chemical analysis. EACs were detectedin 88 % of samples (250 of 285) with limits of quantification(LOQ) ranging from 0.05 to 20 ng/l. Bisphenol A (BPA; LOQ=20 ng/l) was the most frequently detected EAC (66 %) andits predicted no-effect concentration (PNEC) was exceeded 24times. The most common hormone was estrone, detected in28 % of samples (LOQ=1 ng/l), and the PNEC was alsoexceeded 24 times. 17α-Ethinylestradiol (LOQ=0.05 ng/l)was detected in 10 % of samples at concentrations rangingfrom 0.05 to 0.17 ng/l. It was detected in many sampleswith no wastewater influence, and the PNEC wasexceeded 13 times. In parallel to the chemical analysis,endocrine activity was assessed using a battery ofCALUX bioassays. Estrogenic activity was detected in19 % (53 of 285) of samples (LOQ=0.1 ng/l 17β-estradiol equivalent; EEQ). Seven samples exhibitedestrogenic activity (1–6.5 ng/l EEQ) greater than the PNECfor 17β-estradiol. Anti-progestagenic activity was detected in16 % of samples (LOQ=8 ng/l mifepristone equivalents;MifEQ), but the causative compounds are unknown. Withseveral compounds and endocrine activity exceeding PNECvalues, there is potential risk to the Australian freshwaterecosystems.

KW - AR-CALUX . EDC . Endocrine disruption .

KW - ER-CALUX . Ethinylestradiol . PR-CALUX

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DO - 10.1007/s11356-014-3235-7

M3 - Article

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JF - Environmental Science and Pollution Research

SN - 0944-1344

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