Amplification of MMP-2 and MMP-9 production by prostate cancer cell lines via activation of protease-activated receptors

S.R. Wilson, S. Gallagher, K. Warpeha, S.J. Hawthorne

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

BACKGROUND. The matrix metalloproteinases (MMP) are a family of proteolytic enzymes involved in facilitating cancer metastasis. Protease-activated receptors (PARs) have previously been shown to be involved in pathways of MMP upregulation by tumor cells.
METHODS. Two androgen independent prostate cancer cell lines, PC3 and DU-145, and one androgen dependent prostate cancer line LNCaP, were investigated. PAR expression was detected using RT-PCR and immunofluorochemistry (IFC) techniques. MMP activity assays
were used to quantify the levels of MMP-2 and -9 on all three prostate cell lines after PAR activation.
RESULTS. RT-PCR and IFC showed the presence of PAR-1 and PAR-2 in all cell lines investigated, only LNCaP showed PAR-3 and PAR-4 expression. Increased levels of MMP-2 and MMP-9 activity, up to sevenfold depending on prostate cancer cell line, following PAR activation by specific PAR peptides was shown.
CONCLUSION. Preliminary studies show the activation of PAR-1 or PAR-2 produced increased levels of MMP-2 and MMP-9 activity in prostate cancer cell lines, indicating their potential role in the metastasis of prostate cancer cells.
Original languageEnglish
Pages (from-to)168-174
Number of pages7
JournalThe Prostate
Volume60
Issue number2
DOIs
Publication statusPublished - 2004

Keywords

  • protease-activated receptors
  • prostate cancer
  • metastasis
  • matrix metalloproteinases

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