Amplification of MMP-2 and MMP-9 production by prostate cancer cell lines via activation of protease-activated receptors

S.R. Wilson, S. Gallagher, K. Warpeha, S.J. Hawthorne

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

BACKGROUND. The matrix metalloproteinases (MMP) are a family of proteolytic enzymes involved in facilitating cancer metastasis. Protease-activated receptors (PARs) have previously been shown to be involved in pathways of MMP upregulation by tumor cells.
METHODS. Two androgen independent prostate cancer cell lines, PC3 and DU-145, and one androgen dependent prostate cancer line LNCaP, were investigated. PAR expression was detected using RT-PCR and immunofluorochemistry (IFC) techniques. MMP activity assays
were used to quantify the levels of MMP-2 and -9 on all three prostate cell lines after PAR activation.
RESULTS. RT-PCR and IFC showed the presence of PAR-1 and PAR-2 in all cell lines investigated, only LNCaP showed PAR-3 and PAR-4 expression. Increased levels of MMP-2 and MMP-9 activity, up to sevenfold depending on prostate cancer cell line, following PAR activation by specific PAR peptides was shown.
CONCLUSION. Preliminary studies show the activation of PAR-1 or PAR-2 produced increased levels of MMP-2 and MMP-9 activity in prostate cancer cell lines, indicating their potential role in the metastasis of prostate cancer cells.
LanguageEnglish
Pages168-174
Number of pages7
JournalThe Prostate
Volume60
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

Proteinase-Activated Receptors
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Prostatic Neoplasms
Cell Line
Matrix Metalloproteinases
PAR-2 Receptor
PAR-1 Receptor
Androgens
Neoplasm Metastasis
Polymerase Chain Reaction
Prostate
Neoplasms
Peptide Hydrolases
Up-Regulation
Peptides

Keywords

  • protease-activated receptors
  • prostate cancer
  • metastasis
  • matrix metalloproteinases

Cite this

@article{96ed4d71404947b3afaf451a719e158d,
title = "Amplification of MMP-2 and MMP-9 production by prostate cancer cell lines via activation of protease-activated receptors",
abstract = "BACKGROUND. The matrix metalloproteinases (MMP) are a family of proteolytic enzymes involved in facilitating cancer metastasis. Protease-activated receptors (PARs) have previously been shown to be involved in pathways of MMP upregulation by tumor cells.METHODS. Two androgen independent prostate cancer cell lines, PC3 and DU-145, and one androgen dependent prostate cancer line LNCaP, were investigated. PAR expression was detected using RT-PCR and immunofluorochemistry (IFC) techniques. MMP activity assayswere used to quantify the levels of MMP-2 and -9 on all three prostate cell lines after PAR activation.RESULTS. RT-PCR and IFC showed the presence of PAR-1 and PAR-2 in all cell lines investigated, only LNCaP showed PAR-3 and PAR-4 expression. Increased levels of MMP-2 and MMP-9 activity, up to sevenfold depending on prostate cancer cell line, following PAR activation by specific PAR peptides was shown.CONCLUSION. Preliminary studies show the activation of PAR-1 or PAR-2 produced increased levels of MMP-2 and MMP-9 activity in prostate cancer cell lines, indicating their potential role in the metastasis of prostate cancer cells.",
keywords = "protease-activated receptors, prostate cancer, metastasis, matrix metalloproteinases",
author = "S.R. Wilson and S. Gallagher and K. Warpeha and S.J. Hawthorne",
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Amplification of MMP-2 and MMP-9 production by prostate cancer cell lines via activation of protease-activated receptors. / Wilson, S.R.; Gallagher, S.; Warpeha, K.; Hawthorne, S.J.

In: The Prostate, Vol. 60, No. 2, 2004, p. 168-174.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Amplification of MMP-2 and MMP-9 production by prostate cancer cell lines via activation of protease-activated receptors

AU - Wilson, S.R.

AU - Gallagher, S.

AU - Warpeha, K.

AU - Hawthorne, S.J.

N1 - cited By 61

PY - 2004

Y1 - 2004

N2 - BACKGROUND. The matrix metalloproteinases (MMP) are a family of proteolytic enzymes involved in facilitating cancer metastasis. Protease-activated receptors (PARs) have previously been shown to be involved in pathways of MMP upregulation by tumor cells.METHODS. Two androgen independent prostate cancer cell lines, PC3 and DU-145, and one androgen dependent prostate cancer line LNCaP, were investigated. PAR expression was detected using RT-PCR and immunofluorochemistry (IFC) techniques. MMP activity assayswere used to quantify the levels of MMP-2 and -9 on all three prostate cell lines after PAR activation.RESULTS. RT-PCR and IFC showed the presence of PAR-1 and PAR-2 in all cell lines investigated, only LNCaP showed PAR-3 and PAR-4 expression. Increased levels of MMP-2 and MMP-9 activity, up to sevenfold depending on prostate cancer cell line, following PAR activation by specific PAR peptides was shown.CONCLUSION. Preliminary studies show the activation of PAR-1 or PAR-2 produced increased levels of MMP-2 and MMP-9 activity in prostate cancer cell lines, indicating their potential role in the metastasis of prostate cancer cells.

AB - BACKGROUND. The matrix metalloproteinases (MMP) are a family of proteolytic enzymes involved in facilitating cancer metastasis. Protease-activated receptors (PARs) have previously been shown to be involved in pathways of MMP upregulation by tumor cells.METHODS. Two androgen independent prostate cancer cell lines, PC3 and DU-145, and one androgen dependent prostate cancer line LNCaP, were investigated. PAR expression was detected using RT-PCR and immunofluorochemistry (IFC) techniques. MMP activity assayswere used to quantify the levels of MMP-2 and -9 on all three prostate cell lines after PAR activation.RESULTS. RT-PCR and IFC showed the presence of PAR-1 and PAR-2 in all cell lines investigated, only LNCaP showed PAR-3 and PAR-4 expression. Increased levels of MMP-2 and MMP-9 activity, up to sevenfold depending on prostate cancer cell line, following PAR activation by specific PAR peptides was shown.CONCLUSION. Preliminary studies show the activation of PAR-1 or PAR-2 produced increased levels of MMP-2 and MMP-9 activity in prostate cancer cell lines, indicating their potential role in the metastasis of prostate cancer cells.

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KW - metastasis

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