Amphibian skin secretomics: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea: Discovery of a novel peptide family, the hyposins

Alan Hunter Thompson, AJ Bjourson, David Francis Orr, Chris Shaw, Stephen McClean

Research output: Contribution to journalArticle

31 Citations (Scopus)


This study reports the variety of peptides present in the skin secretory peptidome of Phyllomedusa hypochondrialis azurea. Peptide structures, along with post-translational modifications, were elucidated by QTOF MS/MS analysis, cDNA sequencing, or a combination of both. Twenty-two peptides, including 19 novel structures, were identified from six different structural classes, including tryptophyllins, dermorphins, and a novel group of peptides termed hyposins. The study demonstrates the power of this combined approach to mine the rich peptidome compliment of the amphibian defensive skin secretome.
Original languageEnglish
Pages (from-to)3604-3613
JournalJournal of Proteome Research
Issue number9
Publication statusPublished - Sep 2007


Cite this