Abstract
The oxidation of D-[U-C-14[glucose, L-[1-C-14]leucine, L-[U-C-14]leucine, L-[U-C-14]glutamine and L[U-C-14]arginine by BRIN-BD11 cells, which represent a novel insulin-secreting cell line obtained by electrofusion of tumoural and normal islet cells, was compared to the oxidation of the same nutrients in the parent tumoural RINm5F cells. The oxidation of D-[U-C-14]glucose, L[U-C-14]glutamine and L-[U-C-14]arginine by BRIN-BD11 cells was equal to or higher than that found in RINm5F cells, whilst the opposite situation prevailed in the case of C-14-labelled L-leucine. These findings suggest a more efficient contribution of the Krebs cycle to overall ATP generation in the hybrid cells than in the parent tumoural cells.
| Original language | English |
|---|---|
| Pages (from-to) | 691-692 |
| Journal | Medical Science Research |
| Volume | 24 |
| Issue number | 10 |
| Publication status | Published (in print/issue) - Oct 1996 |