Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats

Ghada Alomari; Bahaa Al‐trad; Salehhuddin Hamdan; Alaa A. A. Aljabali; Mazhar Salim Al Zoubi; Khalid Al‐batanyeh; Janti Qar; Gregory J. Eaton; Almuthanna K. Alkaraki; Walhan Alshaer; Saja Haifawi; Khairunadwa Jemon; Dinesh Kumar Chellappan; Kamal Dua; Murtaza M. Tambuwala

doi:10.1049/nbt2.12026

Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats

Ghada Alomari
, Bahaa Al‐trad
, Salehhuddin Hamdan
, Alaa A. A. Aljabali
, Mazhar Salim Al Zoubi
, Khalid Al‐batanyeh
, Janti Qar
, Gregory J. Eaton
, Almuthanna K. Alkaraki
, Walhan Alshaer
, Saja Haifawi
, Khairunadwa Jemon
, Dinesh Kumar Chellappan
, Kamal Dua
, Murtaza M. Tambuwala

Yarmouk University
Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia School of Life Sciences, University of Technology Sydney, Sydney, NSW 2007, Australia
School of Biomedical Sciences and Pharmacy, The University of Newcastle, Callaghan, Australia

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

256 Downloads (Pure)

Abstract

This study examines the effect of nanoparticles with zinc oxides (ZnONPs) on diabetic nephropathy, which is the primary cause of mortality for diabetic patients with end‐stage renal disease. Diabetes in adult male rats was induced via intraperitoneal injection of streptozotocin. ZnONPs were intraperitoneally administered to diabetic rats daily for 7 weeks. Diabetes was associated with increases in blood glucose level, 24‐h urinary albumin excretion rate, glomerular basement membrane thickness, renal oxidative stress markers, and renal mRNA or protein expression of transforming growth factor‐β1, fibronectin, collagen‐IV, tumour necrosis factor‐α and vascular endothelial growth factor‐A. Moreover, the expression of nephrin and podocin, and the mRNA expression of matrix metalloproteinase‐9 were decreased in the diabetic group. These changes were not detected in the control group and were significantly prevented by ZnONP treatment. These results provide evidence that ZnONPs ameliorate the renal damage induced in a diabetic rat model of nephropathy through improving renal functionality; inhibiting renal fibrosis, oxidative stress, inflammation and abnormal angiogenesis; and delaying the development of podocyte injury. The present findings may help design the clinical application of ZnONPs for protection against the development of diabetic nephropathy.

Original language	English
Pages (from-to)	473-483
Number of pages	11
Journal	IET Nanobiotechnology
Volume	15
Issue number	5
Early online date	10 Mar 2021
DOIs	https://doi.org/10.1049/nbt2.12026
Publication status	Published (in print/issue) - 1 Jul 2021

Bibliographical note

Funding Information:
The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit.

Funding Information:
The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit.

Publisher Copyright:
© 2021 The Authors. IET Nanobiotechnology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology.

Funding

Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Publisher Copyright: © 2021 The Authors. IET Nanobiotechnology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biotechnology
Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials

Access to Document

10.1049/nbt2.12026Licence: CC BY

nbt2.12026Final published version, 1.54 MBLicence: CC BY

Cite this

Alomari, G., Al‐trad, B., Hamdan, S., Aljabali, A. A. A., Al Zoubi, M. S., Al‐batanyeh, K., Qar, J., Eaton, G. J., Alkaraki, A. K., Alshaer, W., Haifawi, S., Jemon, K., Chellappan, D. K., Dua, K., & Tambuwala, M. M. (2021). Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats. IET Nanobiotechnology, 15(5), 473-483. https://doi.org/10.1049/nbt2.12026

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title = "Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats",

abstract = "This study examines the effect of nanoparticles with zinc oxides (ZnONPs) on diabetic nephropathy, which is the primary cause of mortality for diabetic patients with end‐stage renal disease. Diabetes in adult male rats was induced via intraperitoneal injection of streptozotocin. ZnONPs were intraperitoneally administered to diabetic rats daily for 7 weeks. Diabetes was associated with increases in blood glucose level, 24‐h urinary albumin excretion rate, glomerular basement membrane thickness, renal oxidative stress markers, and renal mRNA or protein expression of transforming growth factor‐β1, fibronectin, collagen‐IV, tumour necrosis factor‐α and vascular endothelial growth factor‐A. Moreover, the expression of nephrin and podocin, and the mRNA expression of matrix metalloproteinase‐9 were decreased in the diabetic group. These changes were not detected in the control group and were significantly prevented by ZnONP treatment. These results provide evidence that ZnONPs ameliorate the renal damage induced in a diabetic rat model of nephropathy through improving renal functionality; inhibiting renal fibrosis, oxidative stress, inflammation and abnormal angiogenesis; and delaying the development of podocyte injury. The present findings may help design the clinical application of ZnONPs for protection against the development of diabetic nephropathy.",

keywords = "Biotechnology, Electrical and Electronic Engineering, Electronic, Optical and Magnetic Materials",

author = "Ghada Alomari and Bahaa Al‐trad and Salehhuddin Hamdan and Aljabali, \{Alaa A. A.\} and \{Al Zoubi\}, \{Mazhar Salim\} and Khalid Al‐batanyeh and Janti Qar and Eaton, \{Gregory J.\} and Alkaraki, \{Almuthanna K.\} and Walhan Alshaer and Saja Haifawi and Khairunadwa Jemon and Chellappan, \{Dinesh Kumar\} and Kamal Dua and Tambuwala, \{Murtaza M.\}",

note = "Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Publisher Copyright: {\textcopyright} 2021 The Authors. IET Nanobiotechnology published by John Wiley \& Sons Ltd on behalf of The Institution of Engineering and Technology.",

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Alomari, G, Al‐trad, B, Hamdan, S, Aljabali, AAA, Al Zoubi, MS, Al‐batanyeh, K, Qar, J, Eaton, GJ, Alkaraki, AK, Alshaer, W, Haifawi, S, Jemon, K, Chellappan, DK, Dua, K & Tambuwala, MM 2021, 'Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats', IET Nanobiotechnology, vol. 15, no. 5, pp. 473-483. https://doi.org/10.1049/nbt2.12026

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T1 - Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats

AU - Alomari, Ghada

AU - Al‐trad, Bahaa

AU - Hamdan, Salehhuddin

AU - Aljabali, Alaa A. A.

AU - Al Zoubi, Mazhar Salim

AU - Al‐batanyeh, Khalid

AU - Qar, Janti

AU - Eaton, Gregory J.

AU - Alkaraki, Almuthanna K.

AU - Alshaer, Walhan

AU - Haifawi, Saja

AU - Jemon, Khairunadwa

AU - Chellappan, Dinesh Kumar

AU - Dua, Kamal

AU - Tambuwala, Murtaza M.

N1 - Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Funding Information: The Deanship of Scientific Research and Graduate Studies at the University of Yarmouk funded this work (Grant number 34/2017). We sincerely thank Universiti Teknologi Malaysia for facilitating use of the transmission electron microscope unit. Publisher Copyright: © 2021 The Authors. IET Nanobiotechnology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology.

PY - 2021/7/1

Y1 - 2021/7/1

N2 - This study examines the effect of nanoparticles with zinc oxides (ZnONPs) on diabetic nephropathy, which is the primary cause of mortality for diabetic patients with end‐stage renal disease. Diabetes in adult male rats was induced via intraperitoneal injection of streptozotocin. ZnONPs were intraperitoneally administered to diabetic rats daily for 7 weeks. Diabetes was associated with increases in blood glucose level, 24‐h urinary albumin excretion rate, glomerular basement membrane thickness, renal oxidative stress markers, and renal mRNA or protein expression of transforming growth factor‐β1, fibronectin, collagen‐IV, tumour necrosis factor‐α and vascular endothelial growth factor‐A. Moreover, the expression of nephrin and podocin, and the mRNA expression of matrix metalloproteinase‐9 were decreased in the diabetic group. These changes were not detected in the control group and were significantly prevented by ZnONP treatment. These results provide evidence that ZnONPs ameliorate the renal damage induced in a diabetic rat model of nephropathy through improving renal functionality; inhibiting renal fibrosis, oxidative stress, inflammation and abnormal angiogenesis; and delaying the development of podocyte injury. The present findings may help design the clinical application of ZnONPs for protection against the development of diabetic nephropathy.

AB - This study examines the effect of nanoparticles with zinc oxides (ZnONPs) on diabetic nephropathy, which is the primary cause of mortality for diabetic patients with end‐stage renal disease. Diabetes in adult male rats was induced via intraperitoneal injection of streptozotocin. ZnONPs were intraperitoneally administered to diabetic rats daily for 7 weeks. Diabetes was associated with increases in blood glucose level, 24‐h urinary albumin excretion rate, glomerular basement membrane thickness, renal oxidative stress markers, and renal mRNA or protein expression of transforming growth factor‐β1, fibronectin, collagen‐IV, tumour necrosis factor‐α and vascular endothelial growth factor‐A. Moreover, the expression of nephrin and podocin, and the mRNA expression of matrix metalloproteinase‐9 were decreased in the diabetic group. These changes were not detected in the control group and were significantly prevented by ZnONP treatment. These results provide evidence that ZnONPs ameliorate the renal damage induced in a diabetic rat model of nephropathy through improving renal functionality; inhibiting renal fibrosis, oxidative stress, inflammation and abnormal angiogenesis; and delaying the development of podocyte injury. The present findings may help design the clinical application of ZnONPs for protection against the development of diabetic nephropathy.

KW - Biotechnology

KW - Electrical and Electronic Engineering

KW - Electronic, Optical and Magnetic Materials

UR - https://www.scopus.com/pages/publications/85107800146

UR - https://onlinelibrary.wiley.com/doi/10.1049/nbt2.12026

U2 - 10.1049/nbt2.12026

DO - 10.1049/nbt2.12026

M3 - Article

C2 - 34694755

SN - 1751-8741

VL - 15

SP - 473

EP - 483

JO - IET Nanobiotechnology

JF - IET Nanobiotechnology

IS - 5

ER -

Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin‐induced type 1 diabetes in rats

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

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