Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology

Abdelbary Elhissi, Kanar Hidayat, David Phoenix, Enosh Mwesigwa, StJohn Crean, Waqar Ahmed, Ahmed Faheem, Kevin M.G. Taylor

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    The aerosol properties of niosomes were studied using Aeroneb Pro and Omron MicroAir vibrating-mesh nebulizers and Pari LC Sprint air-jet nebulizer. Proniosomes were prepared by coating sucrose particles with Span 60 (sorbitan monostearate), cholesterol and beclometasone dipropionate (BDP) (1:1:0.1). Nano-sized niosomes were produced by manual shaking of the proniosomes in deionized water followed by sonication (median size 236 nm). The entrapment of BDP in proniosome-derived niosomes was higher than that in conventional thin film-made niosomes, being 36.4% and 27.5% respectively. All nebulizers generated aerosols with very high drug output, which was 83.6% using the Aeroneb Pro, 85.5% using the Pari and 72.4% using the Omron. The median droplet size was 3.32 m, 3.06 m and 4.86 m for the Aeroneb Pro, Pari and Omron nebulizers respectively and the “fine particle fraction” (FPF) of BDP was respectively 68.7%, 76.2% and 42.1%. The predicted extrathoracic deposition, based on size distribution of nebulized droplets was negligible for all devices, suggesting all of them are potentially suitable for pulmonary delivery of niosomes. The predicted drug deposition in the alveolar region was low using the Omron (3.2%), but greater using the Aeroneb Pro (17.4%) and the Pari (20.5%). Overall, noisome- BDP aerosols with high drug output and FPF can be generated from proniosomes and delivered using vibrating-mesh or air-jet nebulizers.
    LanguageEnglish
    Pages193-199
    JournalInternational Journal of Pharmaceutics
    Volume444
    Early online date5 Jan 2013
    DOIs
    Publication statusE-pub ahead of print - 5 Jan 2013

    Fingerprint

    Nebulizers and Vaporizers
    Beclomethasone
    Liposomes
    Air
    Technology
    Aerosols
    Pharmaceutical Preparations
    Sonication
    Sucrose
    Cholesterol
    Equipment and Supplies
    Lung
    Water
    sorbitan monostearate

    Keywords

    • Beclometasone dipropionate
    • Liposome
    • Nebulizer
    • Niosome
    • Proniosome

    Cite this

    Elhissi, Abdelbary ; Hidayat, Kanar ; Phoenix, David ; Mwesigwa, Enosh ; Crean, StJohn ; Ahmed, Waqar ; Faheem, Ahmed ; Taylor, Kevin M.G. / Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology. In: International Journal of Pharmaceutics. 2013 ; Vol. 444. pp. 193-199.
    @article{648cb6a4e31b4881938e8c86c8fc28fc,
    title = "Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology",
    abstract = "The aerosol properties of niosomes were studied using Aeroneb Pro and Omron MicroAir vibrating-mesh nebulizers and Pari LC Sprint air-jet nebulizer. Proniosomes were prepared by coating sucrose particles with Span 60 (sorbitan monostearate), cholesterol and beclometasone dipropionate (BDP) (1:1:0.1). Nano-sized niosomes were produced by manual shaking of the proniosomes in deionized water followed by sonication (median size 236 nm). The entrapment of BDP in proniosome-derived niosomes was higher than that in conventional thin film-made niosomes, being 36.4{\%} and 27.5{\%} respectively. All nebulizers generated aerosols with very high drug output, which was 83.6{\%} using the Aeroneb Pro, 85.5{\%} using the Pari and 72.4{\%} using the Omron. The median droplet size was 3.32 m, 3.06 m and 4.86 m for the Aeroneb Pro, Pari and Omron nebulizers respectively and the “fine particle fraction” (FPF) of BDP was respectively 68.7{\%}, 76.2{\%} and 42.1{\%}. The predicted extrathoracic deposition, based on size distribution of nebulized droplets was negligible for all devices, suggesting all of them are potentially suitable for pulmonary delivery of niosomes. The predicted drug deposition in the alveolar region was low using the Omron (3.2{\%}), but greater using the Aeroneb Pro (17.4{\%}) and the Pari (20.5{\%}). Overall, noisome- BDP aerosols with high drug output and FPF can be generated from proniosomes and delivered using vibrating-mesh or air-jet nebulizers.",
    keywords = "Beclometasone dipropionate, Liposome, Nebulizer, Niosome, Proniosome",
    author = "Abdelbary Elhissi and Kanar Hidayat and David Phoenix and Enosh Mwesigwa and StJohn Crean and Waqar Ahmed and Ahmed Faheem and Taylor, {Kevin M.G.}",
    note = "Reference text: Albasarah, Y.Y., Somavarapu, S., Stapleton, P., Taylor, K.M., 2010. Chitosan-coated antifungal formulations for nebulisation. J. Pharm. Pharmacol. 62, 821–828. Abd-Elbary, A., El-Laithy, H.M., Tadros, M.I., 2008. Sucrose stearate-based pronisomes-derived niosomes for the nebulisable delivery of cromolyn sodium. Int. J. Pharm. 357, 189–198. Batavia, R., Taylor, K.M.G., Craig, D.Q.M., Thomas, M., 2001. The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method. Int. J. Pharm. 212, 109–119. Bridges, P.A., Taylor, K.M.G., 2000. The effects of freeze-drying on the stability of liposomes to jet nebulization. J. Pharm. Pharmacol. 53, 393–398. Clark, A.R., 1995. The use of laser diffraction for the evaluation of the aerosol clouds generated by medical nebulizers. Int. J. Pharm. 115, 69–78. Clay, M.M., Pavia, D., Newman, S.P., Lennard-Jones, T., Clarke, S.W., 1983. Assessment of jet nebulisers for lung aerosol therapy. Lancet 2, 592–594. Crommelin, D.J., 1984. Influence of lipid composition and ionic strength on the physical stability of liposomes. J. Pharm. Sci. 73, 1559–1563. Crowe, L.M., Crowe, J.H., 1988. Trehalose and dry dipalmitoylphosphatidylcholine revisted. Biochim. Biophys. Acta 946, 193–201. Dahlbنck, M., 1994. Behavior of nebulizing solutions and suspensions. J. Aerosol Med. 7 (Suppl. 1), S13–S18. Darwis, Y., Kellaway, I.W., 2001. Nebulisation of rehydrated freeze-dried beclomethasone dipropionate liposomes. Int. J. Pharm. 215, 113–121. Desai, T.R., Finlay, W.H., 2002. Nebulization of niosomal all-trans-retinoic acid: an inexpensive alternative to conventional liposomes. Int. J. Pharm. 241, 311–317. Dhand, R., 2002. Nebulizers that use a vibrating mesh or plate with multiple apertures to generate aerosol. Respir. Care 47, 1406–1416. Elhissi, A.M.A., Taylor, K.M.G., 2005. Delivery of liposomes generated from proliposomes using air-jet, ultrasonic and vibrating-mesh nebulisers. J. Drug Del. Sci. Technol. 15, 261–265. Elhissi, A.M.A., Karnam, K.K., Danesh, M.R., Gill, H.S., Taylor, K.M.G., 2006a. Formulations generated from ethanol-based proliposomes for delivery via medical nebulizers. J. Pharm. Pharmacol. 58, 887–894. Elhissi, A.M.A., O’Neill, M.A.A., Roberts, S.A., Taylor, K.M.G., 2006b. A calorimetric study of dimyristoylphosphatidylcholine phase transitions and steroid–liposome interactions for liposomes prepared by thin film and proliposome methods. Int. J. Pharm. 320, 124–130. Elhissi, A.M., Faizi, M., Naji, W.F., Gill, H.S., Taylor, K.M., 2007. Physical stability and aerosol properties of liposomes delivered using an air-jet nebulizer and a novel micropump device with large mesh apertures. Int. J. Pharm. 334, 62–70. Elhissi, A., Gill, H., Ahmed, W., Taylor, K., 2011a. Vibrating-mesh nebulization of liposomes generated using an ethanol-based proliposome technology. J. Liposome Res. 21, 173–180. Elhissi, A.M.A., O’Neill, M., Ahmed, W., Taylor, K.M.G., 2011b. High sensitivity differential scanning calorimetry for measurement of steroid entrapment in nebulised liposomes generated from proliposomes. Micro Nano Lett. 6, 694–697. Ghazanfari, T., Elhissi, A.M., Ding, Z., Taylor, K.M., 2007. The influence of fluid physicochemical properties on vibrating-mesh nebulization. Int. J. Pharm. 339, 103–111. Hao, Y-M., Li, K., 2011. Entrapment and release difference resulting from hydrogen bonding interactions in niosomes. Int. J. Pharm. 403, 245–253. Heyder, J., 1982. Particle transport onto human airway surfaces. Eur. J. Respir. Dis. Suppl. 119, 29–50. Hu, C., Rhodes, D.G., 1999. Proniosomes: a novel drug carrier preparation. Int. J. Pharm. 206, 110–122. Junyaprasert, V.B., Teeranachaideekul, V., Supaperm, T., 2008. Effect of charged and non-ionic membrane additives on physicochemical properties and stability of niosomes. AAPS Pharm. SciTech. 9, 851–859. Kellaway, I.W., Farr, S.J., 1990. Liposomes as drug delivery systems to the lung. Adv. Drug Deliv. Rev. 5, 149–161. Kwong, W.T.J., Ho, S.L., Coates, A.L., 2000. Comparison of nebulized particle size distribution with Malvern laser diffraction analyzer versus Andersen Cascade Impactor and Low-flow Marple Personal Cascade Impactor. J. Aerosol Med. 13, 303–314. Lange, C.F., Hancock, R.E.W., Samuel, J., Finlay, W.H., 2001. In vitro aerosol delivery and regional airway surface liquid concentration of a liposomal cationic peptide. J. Pharm. Sci. 90, 1647–1657. Mosharraf, M., Taylor, K.M., Craig, D.Q., 1995. Effect of calcium ions on the surface charge and aggregation of phosphatidylcholine liposomes. J. Drug Target. 2, 541–545. Nasr, M., Nawaz, S., Elhissi, A.M.A., 2012. Amphotericin B lipid nanoemulsion aerosols for targeting peripheral respiratory airways via nebulization. Int. J. Pharm. 436, 611–616. Newman, S., Gee-Turner, A., 2005. The Omron MicroAir vibrating mesh technology nebuliser, a 21st century approach to inhalation therapy. Drug Deliv. Syst. Sci. 4, 45–48. Niven, R.W., Speer, M., Schreier, H., 1991. Nebulization of liposomes. II. The effects of size and modeling of solute release profiles. Pharm. Res. 8, 217–221. None, L.V., Grimbert, D., Becquemin, M.H., Boissinot, E., Le Pape, A., Lemari{\'e}, E., Diot, P., 2001. Validation of laser diffraction method as a substitute for cascade impaction in the European Project for a nebulizer standard. J. Aerosol. Med. 14, 107–114. O’Callaghan, C., Barry, P.W., 1997. The science of nebulised drug delivery. Thorax 52 (Suppl. 2), S31–S44. Ofir, E., Oren, Y., Adin, A., 2007. Electroflocculation: the effect of zeta-potential on particle size. Desalination 204, 33–38. Payne, N.I., Timmins, P., Ambrose, C.V., Ward, M.D., Ridgway, F., 1986. Proliposomes: a novel solution to an old problem. J. Pharm. Sci. 75, 325–329. Puglia, C., Rizza, L., Drechsler, M., Bonina, F., 2010. Nanoemulsions as vehicles for topical administration of glycyrrhetic acid: characterization and in vitro and in vivo evaluation. Drug Deliv. 17, 123–129. Saari, M., Vidgren, M.T., Koskinen, M.O., Turjanmaa, V.M.H., Nieminen, M.M., 1999. Pulmonary distribution and clearance of two beclomethasone formulations in healthy volunteers. Int. J. Pharm. 181, 1–9. Taylor, K.M.G., Taylor, G., Kellaway, I.W., Stevens, J., 1989. The influence of liposomal encapsulation on sodium cromoglicate pharmacokinetics in man. Pharm. Res. 6, 633–636. Taylor, K.M.G., Taylor, G., Kellaway, I.W., Stevens, J., 1990. The stability of liposomes to nebulisation. Int. J. Pharm. 58, 57–61. Terzano, C., Allegra, L., Alhaique, F., Marianecci, C., Carafa, M., 2005. Nonphospholipid vesicles for pulmonary glucocorticoid delivery. Eur. J. Pharm. Biopharm. 59, 57–62. Uchegbu, I.F., Florence, A.T., 1995. Non-ionic surfactant vesicles (niosomes): physical and pharmaceutical chemistry. Adv. Colloid Interface Sci. 58, 1–55. Uchegbu, I.F., Vyas, S.P., 1998. Non ionic surfactant based vesicles (niosomes) in drug delivery. Int. J. Pharm. 172, 33–70. Van Bommel, E.M.G., Crommelin, D.J.A., 1984. Stability of doxorubicin–liposomes on storage: as an aqueous dispersion, frozen or freeze-dried. Int. J. Pharm. 22, 299–310. Van Winden, E.C.A., Talsma, H., Crommelin, D.J.A., 1998. Thermal analysis of freeze-dried liposome–carbohydrate mixtures with modulated temperature differential scanning calorimetry. J. Pharm. Sci. 87, 231–237. Van Winden, E.C.A., Crommelin, D.J.A., 1997. Long term stability of freeze-dried, lyoprotected doxorubicin liposomes. Eur. J. Pharm. Biopharm. 43, 295–307. Waldrep, J.C., Scherer, P.W., Hess, G.D., Black, M., Knight, V., 1994. Nebulized glucocorticoids in liposomes: aerosol characteristics and human dose estimates. J. Aerosol Med. 7, 135–145. Zaru, M., Mourtas, S., Klepetsanis, P., Fadda, A.M., Antimisiaris, S.G., 2007. Liposomes for drug delivery to the lungs by nebulization. Eur. J. Pharm. Biopharm. 67, 655–666.",
    year = "2013",
    month = "1",
    day = "5",
    doi = "10.1016/j.ijpharm.2012.12.040",
    language = "English",
    volume = "444",
    pages = "193--199",
    journal = "International Journal of Pharmaceutics",
    issn = "0378-5173",
    publisher = "Elsevier",

    }

    Elhissi, A, Hidayat, K, Phoenix, D, Mwesigwa, E, Crean, S, Ahmed, W, Faheem, A & Taylor, KMG 2013, 'Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology', International Journal of Pharmaceutics, vol. 444, pp. 193-199. https://doi.org/10.1016/j.ijpharm.2012.12.040

    Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology. / Elhissi, Abdelbary; Hidayat, Kanar; Phoenix, David; Mwesigwa, Enosh; Crean, StJohn; Ahmed, Waqar; Faheem, Ahmed; Taylor, Kevin M.G.

    In: International Journal of Pharmaceutics, Vol. 444, 05.01.2013, p. 193-199.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Air-jet and vibrating-mesh nebulization of niosomes generated using a particulate-based proniosome technology

    AU - Elhissi, Abdelbary

    AU - Hidayat, Kanar

    AU - Phoenix, David

    AU - Mwesigwa, Enosh

    AU - Crean, StJohn

    AU - Ahmed, Waqar

    AU - Faheem, Ahmed

    AU - Taylor, Kevin M.G.

    N1 - Reference text: Albasarah, Y.Y., Somavarapu, S., Stapleton, P., Taylor, K.M., 2010. Chitosan-coated antifungal formulations for nebulisation. J. Pharm. Pharmacol. 62, 821–828. Abd-Elbary, A., El-Laithy, H.M., Tadros, M.I., 2008. Sucrose stearate-based pronisomes-derived niosomes for the nebulisable delivery of cromolyn sodium. Int. J. Pharm. 357, 189–198. Batavia, R., Taylor, K.M.G., Craig, D.Q.M., Thomas, M., 2001. The measurement of beclomethasone dipropionate entrapment in liposomes: a comparison of a microscope and an HPLC method. Int. J. Pharm. 212, 109–119. Bridges, P.A., Taylor, K.M.G., 2000. The effects of freeze-drying on the stability of liposomes to jet nebulization. J. Pharm. Pharmacol. 53, 393–398. Clark, A.R., 1995. The use of laser diffraction for the evaluation of the aerosol clouds generated by medical nebulizers. Int. J. Pharm. 115, 69–78. Clay, M.M., Pavia, D., Newman, S.P., Lennard-Jones, T., Clarke, S.W., 1983. Assessment of jet nebulisers for lung aerosol therapy. Lancet 2, 592–594. Crommelin, D.J., 1984. Influence of lipid composition and ionic strength on the physical stability of liposomes. J. Pharm. Sci. 73, 1559–1563. Crowe, L.M., Crowe, J.H., 1988. Trehalose and dry dipalmitoylphosphatidylcholine revisted. Biochim. Biophys. Acta 946, 193–201. Dahlbنck, M., 1994. Behavior of nebulizing solutions and suspensions. J. Aerosol Med. 7 (Suppl. 1), S13–S18. Darwis, Y., Kellaway, I.W., 2001. Nebulisation of rehydrated freeze-dried beclomethasone dipropionate liposomes. Int. J. Pharm. 215, 113–121. Desai, T.R., Finlay, W.H., 2002. Nebulization of niosomal all-trans-retinoic acid: an inexpensive alternative to conventional liposomes. Int. J. Pharm. 241, 311–317. Dhand, R., 2002. Nebulizers that use a vibrating mesh or plate with multiple apertures to generate aerosol. Respir. Care 47, 1406–1416. Elhissi, A.M.A., Taylor, K.M.G., 2005. Delivery of liposomes generated from proliposomes using air-jet, ultrasonic and vibrating-mesh nebulisers. J. Drug Del. Sci. Technol. 15, 261–265. Elhissi, A.M.A., Karnam, K.K., Danesh, M.R., Gill, H.S., Taylor, K.M.G., 2006a. Formulations generated from ethanol-based proliposomes for delivery via medical nebulizers. J. Pharm. Pharmacol. 58, 887–894. Elhissi, A.M.A., O’Neill, M.A.A., Roberts, S.A., Taylor, K.M.G., 2006b. A calorimetric study of dimyristoylphosphatidylcholine phase transitions and steroid–liposome interactions for liposomes prepared by thin film and proliposome methods. Int. J. Pharm. 320, 124–130. Elhissi, A.M., Faizi, M., Naji, W.F., Gill, H.S., Taylor, K.M., 2007. Physical stability and aerosol properties of liposomes delivered using an air-jet nebulizer and a novel micropump device with large mesh apertures. Int. J. Pharm. 334, 62–70. Elhissi, A., Gill, H., Ahmed, W., Taylor, K., 2011a. Vibrating-mesh nebulization of liposomes generated using an ethanol-based proliposome technology. J. Liposome Res. 21, 173–180. Elhissi, A.M.A., O’Neill, M., Ahmed, W., Taylor, K.M.G., 2011b. High sensitivity differential scanning calorimetry for measurement of steroid entrapment in nebulised liposomes generated from proliposomes. Micro Nano Lett. 6, 694–697. Ghazanfari, T., Elhissi, A.M., Ding, Z., Taylor, K.M., 2007. The influence of fluid physicochemical properties on vibrating-mesh nebulization. Int. J. Pharm. 339, 103–111. Hao, Y-M., Li, K., 2011. Entrapment and release difference resulting from hydrogen bonding interactions in niosomes. Int. J. Pharm. 403, 245–253. Heyder, J., 1982. Particle transport onto human airway surfaces. Eur. J. Respir. Dis. Suppl. 119, 29–50. Hu, C., Rhodes, D.G., 1999. Proniosomes: a novel drug carrier preparation. Int. J. Pharm. 206, 110–122. Junyaprasert, V.B., Teeranachaideekul, V., Supaperm, T., 2008. Effect of charged and non-ionic membrane additives on physicochemical properties and stability of niosomes. AAPS Pharm. SciTech. 9, 851–859. Kellaway, I.W., Farr, S.J., 1990. Liposomes as drug delivery systems to the lung. Adv. Drug Deliv. Rev. 5, 149–161. Kwong, W.T.J., Ho, S.L., Coates, A.L., 2000. Comparison of nebulized particle size distribution with Malvern laser diffraction analyzer versus Andersen Cascade Impactor and Low-flow Marple Personal Cascade Impactor. J. Aerosol Med. 13, 303–314. Lange, C.F., Hancock, R.E.W., Samuel, J., Finlay, W.H., 2001. In vitro aerosol delivery and regional airway surface liquid concentration of a liposomal cationic peptide. J. Pharm. Sci. 90, 1647–1657. Mosharraf, M., Taylor, K.M., Craig, D.Q., 1995. Effect of calcium ions on the surface charge and aggregation of phosphatidylcholine liposomes. J. Drug Target. 2, 541–545. Nasr, M., Nawaz, S., Elhissi, A.M.A., 2012. Amphotericin B lipid nanoemulsion aerosols for targeting peripheral respiratory airways via nebulization. Int. J. Pharm. 436, 611–616. Newman, S., Gee-Turner, A., 2005. The Omron MicroAir vibrating mesh technology nebuliser, a 21st century approach to inhalation therapy. Drug Deliv. Syst. Sci. 4, 45–48. Niven, R.W., Speer, M., Schreier, H., 1991. Nebulization of liposomes. II. The effects of size and modeling of solute release profiles. Pharm. Res. 8, 217–221. None, L.V., Grimbert, D., Becquemin, M.H., Boissinot, E., Le Pape, A., Lemarié, E., Diot, P., 2001. Validation of laser diffraction method as a substitute for cascade impaction in the European Project for a nebulizer standard. J. Aerosol. Med. 14, 107–114. O’Callaghan, C., Barry, P.W., 1997. The science of nebulised drug delivery. Thorax 52 (Suppl. 2), S31–S44. Ofir, E., Oren, Y., Adin, A., 2007. Electroflocculation: the effect of zeta-potential on particle size. Desalination 204, 33–38. Payne, N.I., Timmins, P., Ambrose, C.V., Ward, M.D., Ridgway, F., 1986. Proliposomes: a novel solution to an old problem. J. Pharm. Sci. 75, 325–329. Puglia, C., Rizza, L., Drechsler, M., Bonina, F., 2010. Nanoemulsions as vehicles for topical administration of glycyrrhetic acid: characterization and in vitro and in vivo evaluation. Drug Deliv. 17, 123–129. Saari, M., Vidgren, M.T., Koskinen, M.O., Turjanmaa, V.M.H., Nieminen, M.M., 1999. Pulmonary distribution and clearance of two beclomethasone formulations in healthy volunteers. Int. J. Pharm. 181, 1–9. Taylor, K.M.G., Taylor, G., Kellaway, I.W., Stevens, J., 1989. The influence of liposomal encapsulation on sodium cromoglicate pharmacokinetics in man. Pharm. Res. 6, 633–636. Taylor, K.M.G., Taylor, G., Kellaway, I.W., Stevens, J., 1990. The stability of liposomes to nebulisation. Int. J. Pharm. 58, 57–61. Terzano, C., Allegra, L., Alhaique, F., Marianecci, C., Carafa, M., 2005. Nonphospholipid vesicles for pulmonary glucocorticoid delivery. Eur. J. Pharm. Biopharm. 59, 57–62. Uchegbu, I.F., Florence, A.T., 1995. Non-ionic surfactant vesicles (niosomes): physical and pharmaceutical chemistry. Adv. Colloid Interface Sci. 58, 1–55. Uchegbu, I.F., Vyas, S.P., 1998. Non ionic surfactant based vesicles (niosomes) in drug delivery. Int. J. Pharm. 172, 33–70. Van Bommel, E.M.G., Crommelin, D.J.A., 1984. Stability of doxorubicin–liposomes on storage: as an aqueous dispersion, frozen or freeze-dried. Int. J. Pharm. 22, 299–310. Van Winden, E.C.A., Talsma, H., Crommelin, D.J.A., 1998. Thermal analysis of freeze-dried liposome–carbohydrate mixtures with modulated temperature differential scanning calorimetry. J. Pharm. Sci. 87, 231–237. Van Winden, E.C.A., Crommelin, D.J.A., 1997. Long term stability of freeze-dried, lyoprotected doxorubicin liposomes. Eur. J. Pharm. Biopharm. 43, 295–307. Waldrep, J.C., Scherer, P.W., Hess, G.D., Black, M., Knight, V., 1994. Nebulized glucocorticoids in liposomes: aerosol characteristics and human dose estimates. J. Aerosol Med. 7, 135–145. Zaru, M., Mourtas, S., Klepetsanis, P., Fadda, A.M., Antimisiaris, S.G., 2007. Liposomes for drug delivery to the lungs by nebulization. Eur. J. Pharm. Biopharm. 67, 655–666.

    PY - 2013/1/5

    Y1 - 2013/1/5

    N2 - The aerosol properties of niosomes were studied using Aeroneb Pro and Omron MicroAir vibrating-mesh nebulizers and Pari LC Sprint air-jet nebulizer. Proniosomes were prepared by coating sucrose particles with Span 60 (sorbitan monostearate), cholesterol and beclometasone dipropionate (BDP) (1:1:0.1). Nano-sized niosomes were produced by manual shaking of the proniosomes in deionized water followed by sonication (median size 236 nm). The entrapment of BDP in proniosome-derived niosomes was higher than that in conventional thin film-made niosomes, being 36.4% and 27.5% respectively. All nebulizers generated aerosols with very high drug output, which was 83.6% using the Aeroneb Pro, 85.5% using the Pari and 72.4% using the Omron. The median droplet size was 3.32 m, 3.06 m and 4.86 m for the Aeroneb Pro, Pari and Omron nebulizers respectively and the “fine particle fraction” (FPF) of BDP was respectively 68.7%, 76.2% and 42.1%. The predicted extrathoracic deposition, based on size distribution of nebulized droplets was negligible for all devices, suggesting all of them are potentially suitable for pulmonary delivery of niosomes. The predicted drug deposition in the alveolar region was low using the Omron (3.2%), but greater using the Aeroneb Pro (17.4%) and the Pari (20.5%). Overall, noisome- BDP aerosols with high drug output and FPF can be generated from proniosomes and delivered using vibrating-mesh or air-jet nebulizers.

    AB - The aerosol properties of niosomes were studied using Aeroneb Pro and Omron MicroAir vibrating-mesh nebulizers and Pari LC Sprint air-jet nebulizer. Proniosomes were prepared by coating sucrose particles with Span 60 (sorbitan monostearate), cholesterol and beclometasone dipropionate (BDP) (1:1:0.1). Nano-sized niosomes were produced by manual shaking of the proniosomes in deionized water followed by sonication (median size 236 nm). The entrapment of BDP in proniosome-derived niosomes was higher than that in conventional thin film-made niosomes, being 36.4% and 27.5% respectively. All nebulizers generated aerosols with very high drug output, which was 83.6% using the Aeroneb Pro, 85.5% using the Pari and 72.4% using the Omron. The median droplet size was 3.32 m, 3.06 m and 4.86 m for the Aeroneb Pro, Pari and Omron nebulizers respectively and the “fine particle fraction” (FPF) of BDP was respectively 68.7%, 76.2% and 42.1%. The predicted extrathoracic deposition, based on size distribution of nebulized droplets was negligible for all devices, suggesting all of them are potentially suitable for pulmonary delivery of niosomes. The predicted drug deposition in the alveolar region was low using the Omron (3.2%), but greater using the Aeroneb Pro (17.4%) and the Pari (20.5%). Overall, noisome- BDP aerosols with high drug output and FPF can be generated from proniosomes and delivered using vibrating-mesh or air-jet nebulizers.

    KW - Beclometasone dipropionate

    KW - Liposome

    KW - Nebulizer

    KW - Niosome

    KW - Proniosome

    U2 - 10.1016/j.ijpharm.2012.12.040

    DO - 10.1016/j.ijpharm.2012.12.040

    M3 - Article

    VL - 444

    SP - 193

    EP - 199

    JO - International Journal of Pharmaceutics

    T2 - International Journal of Pharmaceutics

    JF - International Journal of Pharmaceutics

    SN - 0378-5173

    ER -