Advances in microbial amylases

A Pandey, Poonam Singh - Nee Nigam, CR Soccol, VT Soccol, D Singh, R Mohan

Research output: Contribution to journalArticle

720 Citations (Scopus)

Abstract

This review makes a comprehensive survey of microbial amylases, i.e. alpha-amylase, beta-amylase and glucoamylase. Amylases are among the most important enzymes and are of great significance in present-day biotechnology. Although they can be derived from several sources, such as plants, animals and micro-organisms, the enzymes from microbial sources generally meet industrial demands. Microbial amylases could be potentially useful in the pharmaceutical and fine-chemical industries if enzymes with suitable properties could be prepared. With the advent of new Frontiers in biotechnology, the spectrum of amylase application has widened in many other fields, such as clinical, medicinal and analytical chemistries, as well as their widespread application in starch saccharification and in the textile, food, brewing and distilling industries. In this review, after a brief description of the sources of amylases, we discuss the molecular biology of amylases, describing structures, cloning, sequences, and protoplast fusion and mutagenesis. This is followed by sections on their production and finally the properties of various amylases.
LanguageEnglish
Pages135-152
JournalBiotechnology and Applied Biochemistry
Volume31
Issue numberPart 2
Publication statusPublished - Apr 2000

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Amylases
Biotechnology
Enzymes
beta-Amylase
Glucan 1,4-alpha-Glucosidase
Chemical Industry
Pharmaceutical Chemistry
Protoplasts
Textiles
alpha-Amylases
Mutagenesis
Starch
Organism Cloning
Molecular Biology
Industry
Food
Pharmaceutical Preparations

Cite this

Pandey, A., Singh - Nee Nigam, P., Soccol, CR., Soccol, VT., Singh, D., & Mohan, R. (2000). Advances in microbial amylases. Biotechnology and Applied Biochemistry, 31(Part 2), 135-152.
Pandey, A ; Singh - Nee Nigam, Poonam ; Soccol, CR ; Soccol, VT ; Singh, D ; Mohan, R. / Advances in microbial amylases. In: Biotechnology and Applied Biochemistry. 2000 ; Vol. 31, No. Part 2. pp. 135-152.
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Pandey, A, Singh - Nee Nigam, P, Soccol, CR, Soccol, VT, Singh, D & Mohan, R 2000, 'Advances in microbial amylases', Biotechnology and Applied Biochemistry, vol. 31, no. Part 2, pp. 135-152.

Advances in microbial amylases. / Pandey, A; Singh - Nee Nigam, Poonam; Soccol, CR; Soccol, VT; Singh, D; Mohan, R.

In: Biotechnology and Applied Biochemistry, Vol. 31, No. Part 2, 04.2000, p. 135-152.

Research output: Contribution to journalArticle

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AU - Singh - Nee Nigam, Poonam

AU - Soccol, CR

AU - Soccol, VT

AU - Singh, D

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AB - This review makes a comprehensive survey of microbial amylases, i.e. alpha-amylase, beta-amylase and glucoamylase. Amylases are among the most important enzymes and are of great significance in present-day biotechnology. Although they can be derived from several sources, such as plants, animals and micro-organisms, the enzymes from microbial sources generally meet industrial demands. Microbial amylases could be potentially useful in the pharmaceutical and fine-chemical industries if enzymes with suitable properties could be prepared. With the advent of new Frontiers in biotechnology, the spectrum of amylase application has widened in many other fields, such as clinical, medicinal and analytical chemistries, as well as their widespread application in starch saccharification and in the textile, food, brewing and distilling industries. In this review, after a brief description of the sources of amylases, we discuss the molecular biology of amylases, describing structures, cloning, sequences, and protoplast fusion and mutagenesis. This is followed by sections on their production and finally the properties of various amylases.

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Pandey A, Singh - Nee Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R. Advances in microbial amylases. Biotechnology and Applied Biochemistry. 2000 Apr;31(Part 2):135-152.