Abstract
| Language | English |
|---|---|
| Pages | AAC.02547-16 |
| Journal | Antimicrobial Agents and Chemotherapy |
| Volume | Epub |
| Early online date | 27 Feb 2017 |
| DOIs | |
| Publication status | E-pub ahead of print - 27 Feb 2017 |
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Keywords
- acidic sophorolipids
- microbial pathogens
- growth inhibition
- endothelium
- keratinocytes
- in vivo
- wound healing
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Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing. / Lydon, Helen; Baccile, Niki; Callaghan, Breedge; Marchant, R; Mitchell, CA; Banat, Ibrahim.
In: Antimicrobial Agents and Chemotherapy, Vol. Epub, 27.02.2017, p. AAC.02547-16.Research output: Contribution to journal › Article
TY - JOUR
T1 - Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing
AU - Lydon, Helen
AU - Baccile, Niki
AU - Callaghan, Breedge
AU - Marchant, R
AU - Mitchell, CA
AU - Banat, Ibrahim
PY - 2017/2/27
Y1 - 2017/2/27
N2 - The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (>95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents Enterococcus faecalis and Pseudomonas aeruginosa with significant reduction in c.f.u.'s at concentrations as low as 5 mgmL-1 In addition, the sophorolipid showed similar effects when combined with kanamycin or cefotaxime against the same two bacterial strains. As a potential use of these sophorolipids is as a component of topically applied creams for treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this we evaluated mammalian cell toxicity in vitro using viability tests and revealed no adverse effect on either endothelial or keratinocyte derived cell lines with sophorolipid concentrations
AB - The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (>95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents Enterococcus faecalis and Pseudomonas aeruginosa with significant reduction in c.f.u.'s at concentrations as low as 5 mgmL-1 In addition, the sophorolipid showed similar effects when combined with kanamycin or cefotaxime against the same two bacterial strains. As a potential use of these sophorolipids is as a component of topically applied creams for treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this we evaluated mammalian cell toxicity in vitro using viability tests and revealed no adverse effect on either endothelial or keratinocyte derived cell lines with sophorolipid concentrations
KW - acidic sophorolipids
KW - microbial pathogens
KW - growth inhibition
KW - endothelium
KW - keratinocytes
KW - in vivo
KW - wound healing
U2 - 10.1128/AAC.02547-16
DO - 10.1128/AAC.02547-16
M3 - Article
VL - Epub
SP - AAC.02547-16
JO - Antimicrobial Agents and Chemotherapy
T2 - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
ER -