Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute

Sonam Gupta, Navdeep Raghuwanshi, Ritu Varshney, Ibrahim M Banat, Amit Srivastava, Parul Pruthi, Vikas Pruthi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A potent biosurfactant (BS) producing Bacillus licheniformis SV1 (NCBI GenBank Accession No. KX130852) was isolated from oil contaminated soil sample. Physicochemical investigations (TLC, HPLC, FTIR, GC–MS and NMR) revealed it to be glycolipid in nature. Fibroblast culture assay showed cytocompatibility and increased cell proliferation of 3T3/NIH fibroblast cells treated with this biosurfactant when checked using MTT assay and DAPI fluorescent staining. To evaluate the wound healing potential, BS ointment was formulated and checked for its spreadability and viscosity consistency. In vivo wound healing examination of full thickness skin excision wound rat model demonstrated the prompt re-epithelialization and fibroblast cell proliferation in the early phase while quicker collagen deposition in later phases of wound healing when BS ointment was used. These results validated the potential usage of BS ointment as a transdermal substitute for faster healing of impaired skin wound. Biochemical evaluation also substantiated the highest concentration of hydroxyproline (32.18+-0.46, p <0.001) in the BS ointment treated animal tissue samples compared to the control. Hematoxylin-Eosin (H&E) and Masson's Trichrome staining validated the presence of increased amount of collagen fibers and blood vessels in the test animals treated with BS ointment.
LanguageEnglish
Pages1186-1196
JournalBiomedicine and Pharmacotherapy
Volume94
Early online date18 Aug 2017
DOIs
Publication statusE-pub ahead of print - 18 Aug 2017

Fingerprint

Glycolipids
Ointments
Wound Healing
Fibroblasts
Collagen
Cell Proliferation
Staining and Labeling
Re-Epithelialization
NIH 3T3 Cells
Skin
Hydroxyproline
Nucleic Acid Databases
Wounds and Injuries
Fourier Transform Infrared Spectroscopy
Hematoxylin
Eosine Yellowish-(YS)
Viscosity
Blood Vessels
Oils
Soil

Keywords

  • Biosurfactant
  • Wound healing
  • Fibroblast cell culture
  • MTT
  • DAPI

Cite this

Gupta, Sonam ; Raghuwanshi, Navdeep ; Varshney, Ritu ; Banat, Ibrahim M ; Srivastava, Amit ; Pruthi, Parul ; Pruthi, Vikas. / Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute. In: Biomedicine and Pharmacotherapy. 2017 ; Vol. 94. pp. 1186-1196.
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Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute. / Gupta, Sonam; Raghuwanshi, Navdeep; Varshney, Ritu; Banat, Ibrahim M; Srivastava, Amit; Pruthi, Parul; Pruthi, Vikas.

In: Biomedicine and Pharmacotherapy, Vol. 94, 18.08.2017, p. 1186-1196.

Research output: Contribution to journalArticle

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AU - Gupta, Sonam

AU - Raghuwanshi, Navdeep

AU - Varshney, Ritu

AU - Banat, Ibrahim M

AU - Srivastava, Amit

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AU - Pruthi, Vikas

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