Ac3IV, a V1a and V1b receptor selective vasopressin analogue, protects against hydrocortisone-induced changes in pancreatic islet cell lineage

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Abstract

The Avpr1a (V1a) and Avpr1b (V1b) receptor selective, vasopressin (AVP) analogue, Ac3IV has been shown to improve metabolism and pancreatic islet structure in diabetes and insulin resistance. The present study further investigates these actions by assessing the ability of Ac3IV to protect against pancreatic islet architectural disturbances induced by hydrocortisone (HC) treatment in transgenic Ins1 ;Rosa26-eYFP mice, that possess beta-cell lineage tracing capabilities. HC intervention increased (p < 0.001) energy intake but reduced (p < 0.01) body weight gain, with no impact of Ac3IV. All HC mice had reduced (p < 0.05) circulating glucose, but plasma insulin and glucagon concentrations remained unchanged. However, HC mice presented with increased (p < 0.001) pancreatic insulin content, which was further augmented by Ac3IV. In addition, Ac3IV treatment countered HC-induced increases in islet-, beta- and alpha-cell areas (p < 0.01), as well as promoting islet number towards control levels. This was accompanied by reduced (p < 0.05) beta-cell growth, but enhanced (p < 0.001) alpha-cell proliferation. There were no changes in islet cell apoptotic rates in any of the groups of HC mice, but co-expression of CK19 with insulin in pancreatic ductal cells was reduced by Ac3IV. Assessment of beta-cell lineage revealed that Ac3IV partially protected against HC-mediated de-differentiation of mature beta-cells, whilst also decreasing (p < 0.01) beta- to alpha-cell transdifferentiation. Our data indicate that sustained activation of V1a and V1b receptors exerts positive islet cell transition effects to help retain beta-cell identity in HC mice. [Abstract copyright: Copyright © 2022 Elsevier Inc. All rights reserved.]
Original languageEnglish
Article number170772
Pages (from-to)170772
Number of pages7
JournalPeptides
Volume152
Early online date22 Feb 2022
DOIs
Publication statusE-pub ahead of print - 22 Feb 2022

Bibliographical note

Funding Information:
This work was supported by an Ulster University Vice-Chancellors Ph. D. Research Scholarship to SM , an early career research award from Diabetes UK to RCM and Ulster University selective research funding.

Publisher Copyright:
© 2022 Elsevier Inc.

Keywords

  • vasopressin
  • Hydrocortisone
  • transdifferentiation
  • beta-cell
  • Beta-cell
  • Transdifferentiation
  • Vasopressin

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