A white clover nodulin gene, dd23b, encoding a cysteine cluster protein, is expressed in roots during the very early stages of interaction with Rhizobium leguminosarum biovar trifolii and after treatment with chitolipooligosaccharide Nod factors

MA Crockard, AJ Bjourson, FB Dazzo, JE Cooper

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14 Citations (Scopus)

Abstract

An early nodulin cDNA, dd23b, was isolated from white clover root tissue by differential display RTPCR. Its full-length sequence of 340 nucleotides encodes a predicted 72-amino-acid protein of molecular mass 8.3 kDa, with a polypeptide region containing cysteine pairs spaced in the manner of a cysteine cluster protein. This feature, which is shared by some other late and early nodulins from pea and broad bean, suggests a role in metal ion binding and membrane transport. Temporal and spatial expression patterns were determined during infection and nodulation by the homologous microsymbiont. No expression was found in unchallenged root tissue over a 7-day sampling period. Expression was first detectable in roots by RT-PCR 6 h post-inoculation with Rhizobium leguminosarum biovar trifolii, placing dd23b among the earliest nodulins to be detected to date. In root nodules, expression occurred primarily in the central symbiotic zone, but also in some host cells within the infection zone. Addition of purified wildtype chitolipooligosaccharide Nod factor to axenic white clover roots induced dd23b expression, providing further evidence for the role of this gene in the early plant response to infection by rhizobia.
LanguageEnglish
Pages439-447
JournalJournal of Plant Research
Volume115
Issue number1122
DOIs
Publication statusPublished - Dec 2002

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Rhizobium leguminosarum bv. trifolii
nodulins
Trifolium repens
cysteine
genes
proteins
reverse transcriptase polymerase chain reaction
infection
microsymbionts
root nodules
faba beans
nodulation
metal ions
Rhizobium
plant response
polypeptides
peas
molecular weight
nucleotide sequences
amino acids

Cite this

@article{a77dd1d439284d428bb37c2ca5e5233b,
title = "A white clover nodulin gene, dd23b, encoding a cysteine cluster protein, is expressed in roots during the very early stages of interaction with Rhizobium leguminosarum biovar trifolii and after treatment with chitolipooligosaccharide Nod factors",
abstract = "An early nodulin cDNA, dd23b, was isolated from white clover root tissue by differential display RTPCR. Its full-length sequence of 340 nucleotides encodes a predicted 72-amino-acid protein of molecular mass 8.3 kDa, with a polypeptide region containing cysteine pairs spaced in the manner of a cysteine cluster protein. This feature, which is shared by some other late and early nodulins from pea and broad bean, suggests a role in metal ion binding and membrane transport. Temporal and spatial expression patterns were determined during infection and nodulation by the homologous microsymbiont. No expression was found in unchallenged root tissue over a 7-day sampling period. Expression was first detectable in roots by RT-PCR 6 h post-inoculation with Rhizobium leguminosarum biovar trifolii, placing dd23b among the earliest nodulins to be detected to date. In root nodules, expression occurred primarily in the central symbiotic zone, but also in some host cells within the infection zone. Addition of purified wildtype chitolipooligosaccharide Nod factor to axenic white clover roots induced dd23b expression, providing further evidence for the role of this gene in the early plant response to infection by rhizobia.",
author = "MA Crockard and AJ Bjourson and FB Dazzo and JE Cooper",
year = "2002",
month = "12",
doi = "10.1007/s10265-002-0053-7",
language = "English",
volume = "115",
pages = "439--447",
journal = "Journal of Plant Research",
issn = "0918-9440",
number = "1122",

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TY - JOUR

T1 - A white clover nodulin gene, dd23b, encoding a cysteine cluster protein, is expressed in roots during the very early stages of interaction with Rhizobium leguminosarum biovar trifolii and after treatment with chitolipooligosaccharide Nod factors

AU - Crockard, MA

AU - Bjourson, AJ

AU - Dazzo, FB

AU - Cooper, JE

PY - 2002/12

Y1 - 2002/12

N2 - An early nodulin cDNA, dd23b, was isolated from white clover root tissue by differential display RTPCR. Its full-length sequence of 340 nucleotides encodes a predicted 72-amino-acid protein of molecular mass 8.3 kDa, with a polypeptide region containing cysteine pairs spaced in the manner of a cysteine cluster protein. This feature, which is shared by some other late and early nodulins from pea and broad bean, suggests a role in metal ion binding and membrane transport. Temporal and spatial expression patterns were determined during infection and nodulation by the homologous microsymbiont. No expression was found in unchallenged root tissue over a 7-day sampling period. Expression was first detectable in roots by RT-PCR 6 h post-inoculation with Rhizobium leguminosarum biovar trifolii, placing dd23b among the earliest nodulins to be detected to date. In root nodules, expression occurred primarily in the central symbiotic zone, but also in some host cells within the infection zone. Addition of purified wildtype chitolipooligosaccharide Nod factor to axenic white clover roots induced dd23b expression, providing further evidence for the role of this gene in the early plant response to infection by rhizobia.

AB - An early nodulin cDNA, dd23b, was isolated from white clover root tissue by differential display RTPCR. Its full-length sequence of 340 nucleotides encodes a predicted 72-amino-acid protein of molecular mass 8.3 kDa, with a polypeptide region containing cysteine pairs spaced in the manner of a cysteine cluster protein. This feature, which is shared by some other late and early nodulins from pea and broad bean, suggests a role in metal ion binding and membrane transport. Temporal and spatial expression patterns were determined during infection and nodulation by the homologous microsymbiont. No expression was found in unchallenged root tissue over a 7-day sampling period. Expression was first detectable in roots by RT-PCR 6 h post-inoculation with Rhizobium leguminosarum biovar trifolii, placing dd23b among the earliest nodulins to be detected to date. In root nodules, expression occurred primarily in the central symbiotic zone, but also in some host cells within the infection zone. Addition of purified wildtype chitolipooligosaccharide Nod factor to axenic white clover roots induced dd23b expression, providing further evidence for the role of this gene in the early plant response to infection by rhizobia.

U2 - 10.1007/s10265-002-0053-7

DO - 10.1007/s10265-002-0053-7

M3 - Article

VL - 115

SP - 439

EP - 447

JO - Journal of Plant Research

T2 - Journal of Plant Research

JF - Journal of Plant Research

SN - 0918-9440

IS - 1122

ER -