A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS

Stephen McClean, T MAXWELL, VN RAMACHANDRAN, E O'KANE, Franklin Smyth

Research output: Contribution to journalArticle

Abstract

The differential pulse polarographic (DPP) behavior of SK&F 95601 (I) has been studied in the pH range 0 to 14. At pH less than or equal to 2, (I) rapidly degrades into the sulfenamide (D-1(I)) and sulfide (D-2(I)), which are both reduced by 2e(-) processes, the former being subject to strong reactant adsorption. SK&F 95601 itself is reduced in a 4e process up to pH10, and either pK(2) or pK(3) values, determined spectrophotometrically, are manifested polarographically at neutral pH. A pK(5) value of 9.0 corresponding to the benzimidazole N-H group deprotonation is manifested polarographically with a pK'(5) value of ca. 11. SK&F 95601 and its degradation products D-1(I), degraded D-1(I), and D-2(I) can all be determined by adsorptive stripping voltammetry (AdSV) with limit of detections (LODs) of 10(-8) mol dm(-3), except for the latter molecule with an LOD of 10(-7) mol dm(-3). These LODs are superior to those determined by DPP. Mixtures of these molecules can also be resolved with similar LODs. Cd2+ can be determined by ASV with a superior LOD in the presence of (I), i.e., 0.08 ppb as compared to 0.57 ppb in its absence, due to chelation phenomena.
LanguageEnglish
Pages996-1003
JournalElectroanalysis
Volume6
Issue number11-12
Publication statusPublished - Nov 1994

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Corrosion inhibitors
Adenosine Triphosphatases
Degradation
Deprotonation
Molecules
Sulfides
Voltammetry
Chelation
Adsorption
SK&F 95601
benzimidazole
sulfenamide

Cite this

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title = "A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS",
abstract = "The differential pulse polarographic (DPP) behavior of SK&F 95601 (I) has been studied in the pH range 0 to 14. At pH less than or equal to 2, (I) rapidly degrades into the sulfenamide (D-1(I)) and sulfide (D-2(I)), which are both reduced by 2e(-) processes, the former being subject to strong reactant adsorption. SK&F 95601 itself is reduced in a 4e process up to pH10, and either pK(2) or pK(3) values, determined spectrophotometrically, are manifested polarographically at neutral pH. A pK(5) value of 9.0 corresponding to the benzimidazole N-H group deprotonation is manifested polarographically with a pK'(5) value of ca. 11. SK&F 95601 and its degradation products D-1(I), degraded D-1(I), and D-2(I) can all be determined by adsorptive stripping voltammetry (AdSV) with limit of detections (LODs) of 10(-8) mol dm(-3), except for the latter molecule with an LOD of 10(-7) mol dm(-3). These LODs are superior to those determined by DPP. Mixtures of these molecules can also be resolved with similar LODs. Cd2+ can be determined by ASV with a superior LOD in the presence of (I), i.e., 0.08 ppb as compared to 0.57 ppb in its absence, due to chelation phenomena.",
author = "Stephen McClean and T MAXWELL and VN RAMACHANDRAN and E O'KANE and Franklin Smyth",
year = "1994",
month = "11",
language = "English",
volume = "6",
pages = "996--1003",
journal = "Electroanalysis",
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A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS. / McClean, Stephen; MAXWELL, T; RAMACHANDRAN, VN; O'KANE, E; Smyth, Franklin.

In: Electroanalysis, Vol. 6, No. 11-12, 11.1994, p. 996-1003.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS

AU - McClean, Stephen

AU - MAXWELL, T

AU - RAMACHANDRAN, VN

AU - O'KANE, E

AU - Smyth, Franklin

PY - 1994/11

Y1 - 1994/11

N2 - The differential pulse polarographic (DPP) behavior of SK&F 95601 (I) has been studied in the pH range 0 to 14. At pH less than or equal to 2, (I) rapidly degrades into the sulfenamide (D-1(I)) and sulfide (D-2(I)), which are both reduced by 2e(-) processes, the former being subject to strong reactant adsorption. SK&F 95601 itself is reduced in a 4e process up to pH10, and either pK(2) or pK(3) values, determined spectrophotometrically, are manifested polarographically at neutral pH. A pK(5) value of 9.0 corresponding to the benzimidazole N-H group deprotonation is manifested polarographically with a pK'(5) value of ca. 11. SK&F 95601 and its degradation products D-1(I), degraded D-1(I), and D-2(I) can all be determined by adsorptive stripping voltammetry (AdSV) with limit of detections (LODs) of 10(-8) mol dm(-3), except for the latter molecule with an LOD of 10(-7) mol dm(-3). These LODs are superior to those determined by DPP. Mixtures of these molecules can also be resolved with similar LODs. Cd2+ can be determined by ASV with a superior LOD in the presence of (I), i.e., 0.08 ppb as compared to 0.57 ppb in its absence, due to chelation phenomena.

AB - The differential pulse polarographic (DPP) behavior of SK&F 95601 (I) has been studied in the pH range 0 to 14. At pH less than or equal to 2, (I) rapidly degrades into the sulfenamide (D-1(I)) and sulfide (D-2(I)), which are both reduced by 2e(-) processes, the former being subject to strong reactant adsorption. SK&F 95601 itself is reduced in a 4e process up to pH10, and either pK(2) or pK(3) values, determined spectrophotometrically, are manifested polarographically at neutral pH. A pK(5) value of 9.0 corresponding to the benzimidazole N-H group deprotonation is manifested polarographically with a pK'(5) value of ca. 11. SK&F 95601 and its degradation products D-1(I), degraded D-1(I), and D-2(I) can all be determined by adsorptive stripping voltammetry (AdSV) with limit of detections (LODs) of 10(-8) mol dm(-3), except for the latter molecule with an LOD of 10(-7) mol dm(-3). These LODs are superior to those determined by DPP. Mixtures of these molecules can also be resolved with similar LODs. Cd2+ can be determined by ASV with a superior LOD in the presence of (I), i.e., 0.08 ppb as compared to 0.57 ppb in its absence, due to chelation phenomena.

M3 - Article

VL - 6

SP - 996

EP - 1003

JO - Electroanalysis

T2 - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 11-12

ER -