The differential pulse polarographic (DPP) behavior of SK&F 95601 (I) has been studied in the pH range 0 to 14. At pH less than or equal to 2, (I) rapidly degrades into the sulfenamide (D-1(I)) and sulfide (D-2(I)), which are both reduced by 2e(-) processes, the former being subject to strong reactant adsorption. SK&F 95601 itself is reduced in a 4e process up to pH10, and either pK(2) or pK(3) values, determined spectrophotometrically, are manifested polarographically at neutral pH. A pK(5) value of 9.0 corresponding to the benzimidazole N-H group deprotonation is manifested polarographically with a pK'(5) value of ca. 11. SK&F 95601 and its degradation products D-1(I), degraded D-1(I), and D-2(I) can all be determined by adsorptive stripping voltammetry (AdSV) with limit of detections (LODs) of 10(-8) mol dm(-3), except for the latter molecule with an LOD of 10(-7) mol dm(-3). These LODs are superior to those determined by DPP. Mixtures of these molecules can also be resolved with similar LODs. Cd2+ can be determined by ASV with a superior LOD in the presence of (I), i.e., 0.08 ppb as compared to 0.57 ppb in its absence, due to chelation phenomena.
|Publication status||Published - Nov 1994|
McClean, S., MAXWELL, T., RAMACHANDRAN, VN., O'KANE, E., & Smyth, F. (1994). A STUDY OF THE DIFFERENTIAL-PULSE POLAROGRAPHIC AND STRIPPING VOLTAMETRIC BEHAVIOR OF THE H+/K+ ATPASE INHIBITOR SK-AND-F-95601 AND ITS DEGRADATION PRODUCTS. Electroanalysis, 6(11-12), 996-1003.