A study of the analytical behaviour of selected new molecular entities using electrospray ionisation ion trap mass spectrometry, liquid chromatography, gas chromatography and polarography and their determination in serum at therapeutic concentrations

Virginia Rodriguez Robledo, Franklin Smyth

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    This paper provides analytical chemical information on selected new molecular entities (NMEs) which are drugs that have recently been approved by the FDA. These are the antiretroviral drugs, atazanavir, indinavir and emtricitabine, the antibacterial gemifloxacin, rosuvastatine which is a cholesterol-lowing drug, the anti-cancer drug gefitinib and aprepitant for neurological disorders. Electrospray ionisation-quadrupole ion trap mass spectrometry (ESI-MSn) was employed to generate tandem mass spectrometric (MS2) data of the drugs studied and structural assignments of product ions were supported by quadrupole time-of-flight mass spectrometry (QToF-MS/MS). These fragmentation studies were then utilised in the development and validation of a specific and sensitive liquid chromatographic method (LC-ESI-MS2) to identify and determine these drugs at therapeutic concentration levels in serum after a single protein precipitation procedure with acetonitrile. in addition, this method was compared to the application of gas liquid chromatography-flame ionisation detection (GLC-FID) and differential pulse polarography (DPP) for the analysis of these NMEs in serum. (C) 2008 Elsevier B.V. All rights reserved.
    LanguageEnglish
    Pages221-230
    JournalAnalytica Chimica Acta
    Volume623
    Issue number2
    DOIs
    Publication statusPublished - Aug 2008

    Fingerprint

    Polarographic analysis
    Electrospray ionization
    Liquid chromatography
    Gas chromatography
    Mass spectrometry
    Ions
    Pharmaceutical Preparations
    aprepitant
    Indinavir
    Ionization
    Cholesterol
    Liquids
    Proteins

    Cite this

    @article{d463420a53dc415a9afde914f03202f9,
    title = "A study of the analytical behaviour of selected new molecular entities using electrospray ionisation ion trap mass spectrometry, liquid chromatography, gas chromatography and polarography and their determination in serum at therapeutic concentrations",
    abstract = "This paper provides analytical chemical information on selected new molecular entities (NMEs) which are drugs that have recently been approved by the FDA. These are the antiretroviral drugs, atazanavir, indinavir and emtricitabine, the antibacterial gemifloxacin, rosuvastatine which is a cholesterol-lowing drug, the anti-cancer drug gefitinib and aprepitant for neurological disorders. Electrospray ionisation-quadrupole ion trap mass spectrometry (ESI-MSn) was employed to generate tandem mass spectrometric (MS2) data of the drugs studied and structural assignments of product ions were supported by quadrupole time-of-flight mass spectrometry (QToF-MS/MS). These fragmentation studies were then utilised in the development and validation of a specific and sensitive liquid chromatographic method (LC-ESI-MS2) to identify and determine these drugs at therapeutic concentration levels in serum after a single protein precipitation procedure with acetonitrile. in addition, this method was compared to the application of gas liquid chromatography-flame ionisation detection (GLC-FID) and differential pulse polarography (DPP) for the analysis of these NMEs in serum. (C) 2008 Elsevier B.V. All rights reserved.",
    author = "{Rodriguez Robledo}, Virginia and Franklin Smyth",
    year = "2008",
    month = "8",
    doi = "10.1016/j.aca.2008.06.025",
    language = "English",
    volume = "623",
    pages = "221--230",
    journal = "Analytica Chimica Acta",
    issn = "0003-2670",
    publisher = "Elsevier",
    number = "2",

    }

    TY - JOUR

    T1 - A study of the analytical behaviour of selected new molecular entities using electrospray ionisation ion trap mass spectrometry, liquid chromatography, gas chromatography and polarography and their determination in serum at therapeutic concentrations

    AU - Rodriguez Robledo, Virginia

    AU - Smyth, Franklin

    PY - 2008/8

    Y1 - 2008/8

    N2 - This paper provides analytical chemical information on selected new molecular entities (NMEs) which are drugs that have recently been approved by the FDA. These are the antiretroviral drugs, atazanavir, indinavir and emtricitabine, the antibacterial gemifloxacin, rosuvastatine which is a cholesterol-lowing drug, the anti-cancer drug gefitinib and aprepitant for neurological disorders. Electrospray ionisation-quadrupole ion trap mass spectrometry (ESI-MSn) was employed to generate tandem mass spectrometric (MS2) data of the drugs studied and structural assignments of product ions were supported by quadrupole time-of-flight mass spectrometry (QToF-MS/MS). These fragmentation studies were then utilised in the development and validation of a specific and sensitive liquid chromatographic method (LC-ESI-MS2) to identify and determine these drugs at therapeutic concentration levels in serum after a single protein precipitation procedure with acetonitrile. in addition, this method was compared to the application of gas liquid chromatography-flame ionisation detection (GLC-FID) and differential pulse polarography (DPP) for the analysis of these NMEs in serum. (C) 2008 Elsevier B.V. All rights reserved.

    AB - This paper provides analytical chemical information on selected new molecular entities (NMEs) which are drugs that have recently been approved by the FDA. These are the antiretroviral drugs, atazanavir, indinavir and emtricitabine, the antibacterial gemifloxacin, rosuvastatine which is a cholesterol-lowing drug, the anti-cancer drug gefitinib and aprepitant for neurological disorders. Electrospray ionisation-quadrupole ion trap mass spectrometry (ESI-MSn) was employed to generate tandem mass spectrometric (MS2) data of the drugs studied and structural assignments of product ions were supported by quadrupole time-of-flight mass spectrometry (QToF-MS/MS). These fragmentation studies were then utilised in the development and validation of a specific and sensitive liquid chromatographic method (LC-ESI-MS2) to identify and determine these drugs at therapeutic concentration levels in serum after a single protein precipitation procedure with acetonitrile. in addition, this method was compared to the application of gas liquid chromatography-flame ionisation detection (GLC-FID) and differential pulse polarography (DPP) for the analysis of these NMEs in serum. (C) 2008 Elsevier B.V. All rights reserved.

    U2 - 10.1016/j.aca.2008.06.025

    DO - 10.1016/j.aca.2008.06.025

    M3 - Article

    VL - 623

    SP - 221

    EP - 230

    JO - Analytica Chimica Acta

    T2 - Analytica Chimica Acta

    JF - Analytica Chimica Acta

    SN - 0003-2670

    IS - 2

    ER -