A study of anti-cancer effects of Funalia trogii in vitro and in vivo

Jenny Worthington, Stephanie McKeown, Ibrahim Banat

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Extracts of natural products have been used for many years for health benefits. We report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, Funalia trogii. A variety of biological assays were used to show that a 4 h exposure of HT29, LNCaP, PC3, MCF-7 and MDA-MB-231 tumour cells to extract (0.5–5.0 mg/ml) resulted in significant cytotoxicity. In a clonogenic assay, IC50 values were found to range from 0.4–0.72 mg/ml; exposing fibroblast cells to the extract resulted in no cell kill. The extract resulted in significant cell kill in proliferating endothelial cells but had no toxicity to quiescent cells, this is useful in targeting tumour tissue since endothelial cells in tumours proliferate more rapidly that those found in other parts of the body. When tumours grown in immune compromised mice were injected intratumourally with extract (5 mg/ml twice a week for twoweeks), a 9 day tumour growth delay was observed. The results indicate that the mycelial extract of F. trogii has a promising anti-tumour property.
LanguageEnglish
Pages1477-1483
JournalFood and Chemical Toxicology
Volume49
Issue number7
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Tumors
extracts
neoplasms
Neoplasms
Endothelial cells
Cell Extracts
endothelial cells
Assays
Endothelial Cells
Cells
Basidiomycota
Insurance Benefits
cells
Fibroblasts
Cytotoxicity
in vivo studies
antineoplastic activity
In Vitro Techniques
Biological Products
Human Body

Cite this

Worthington, Jenny ; McKeown, Stephanie ; Banat, Ibrahim. / A study of anti-cancer effects of Funalia trogii in vitro and in vivo. In: Food and Chemical Toxicology. 2011 ; Vol. 49, No. 7. pp. 1477-1483.
@article{7427076e3101413aaddf7d420f31b9c6,
title = "A study of anti-cancer effects of Funalia trogii in vitro and in vivo",
abstract = "Extracts of natural products have been used for many years for health benefits. We report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, Funalia trogii. A variety of biological assays were used to show that a 4 h exposure of HT29, LNCaP, PC3, MCF-7 and MDA-MB-231 tumour cells to extract (0.5–5.0 mg/ml) resulted in significant cytotoxicity. In a clonogenic assay, IC50 values were found to range from 0.4–0.72 mg/ml; exposing fibroblast cells to the extract resulted in no cell kill. The extract resulted in significant cell kill in proliferating endothelial cells but had no toxicity to quiescent cells, this is useful in targeting tumour tissue since endothelial cells in tumours proliferate more rapidly that those found in other parts of the body. When tumours grown in immune compromised mice were injected intratumourally with extract (5 mg/ml twice a week for twoweeks), a 9 day tumour growth delay was observed. The results indicate that the mycelial extract of F. trogii has a promising anti-tumour property.",
author = "Jenny Worthington and Stephanie McKeown and Ibrahim Banat",
year = "2011",
month = "7",
doi = "10.1016/j.fct.2011.02.008",
language = "English",
volume = "49",
pages = "1477--1483",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier",
number = "7",

}

A study of anti-cancer effects of Funalia trogii in vitro and in vivo. / Worthington, Jenny; McKeown, Stephanie; Banat, Ibrahim.

In: Food and Chemical Toxicology, Vol. 49, No. 7, 07.2011, p. 1477-1483.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A study of anti-cancer effects of Funalia trogii in vitro and in vivo

AU - Worthington, Jenny

AU - McKeown, Stephanie

AU - Banat, Ibrahim

PY - 2011/7

Y1 - 2011/7

N2 - Extracts of natural products have been used for many years for health benefits. We report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, Funalia trogii. A variety of biological assays were used to show that a 4 h exposure of HT29, LNCaP, PC3, MCF-7 and MDA-MB-231 tumour cells to extract (0.5–5.0 mg/ml) resulted in significant cytotoxicity. In a clonogenic assay, IC50 values were found to range from 0.4–0.72 mg/ml; exposing fibroblast cells to the extract resulted in no cell kill. The extract resulted in significant cell kill in proliferating endothelial cells but had no toxicity to quiescent cells, this is useful in targeting tumour tissue since endothelial cells in tumours proliferate more rapidly that those found in other parts of the body. When tumours grown in immune compromised mice were injected intratumourally with extract (5 mg/ml twice a week for twoweeks), a 9 day tumour growth delay was observed. The results indicate that the mycelial extract of F. trogii has a promising anti-tumour property.

AB - Extracts of natural products have been used for many years for health benefits. We report on an in vitro and in vivo study into the anti-tumour efficacy of an aqueous extract of the mycelial form of basidiomycete, Funalia trogii. A variety of biological assays were used to show that a 4 h exposure of HT29, LNCaP, PC3, MCF-7 and MDA-MB-231 tumour cells to extract (0.5–5.0 mg/ml) resulted in significant cytotoxicity. In a clonogenic assay, IC50 values were found to range from 0.4–0.72 mg/ml; exposing fibroblast cells to the extract resulted in no cell kill. The extract resulted in significant cell kill in proliferating endothelial cells but had no toxicity to quiescent cells, this is useful in targeting tumour tissue since endothelial cells in tumours proliferate more rapidly that those found in other parts of the body. When tumours grown in immune compromised mice were injected intratumourally with extract (5 mg/ml twice a week for twoweeks), a 9 day tumour growth delay was observed. The results indicate that the mycelial extract of F. trogii has a promising anti-tumour property.

U2 - 10.1016/j.fct.2011.02.008

DO - 10.1016/j.fct.2011.02.008

M3 - Article

VL - 49

SP - 1477

EP - 1483

JO - Food and Chemical Toxicology

T2 - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 7

ER -