A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Sofija Andjelic; Xhevat Lumi; Zoltán Veréb; Natasha Josifovska; Andrea Facskó; Marko Hawlina; Goran Petrovski

doi:10.1155/2014/232659

A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Sofija Andjelic, Xhevat Lumi, Zoltán Veréb, Natasha Josifovska, Andrea Facskó, Marko Hawlina, Goran Petrovski

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

Original language	English
Pages (from-to)	1-10
Journal	Journal of immunology research
Volume	2014
DOIs	https://doi.org/10.1155/2014/232659
Publication status	Published (in print/issue) - 4 Sept 2014

Keywords

viscoelastic
anterior lens capsule
lens epithelial cells
fibrovascular epiretinal membranes
proliferative diabetic retinopathy
calcium imaging
acetylcholine
mechanostimulation
inflammation
IL-6
IL-8
TNFa

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1155/2014/232659

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@article{3ef24cc7bacd447b87008565146aac56,

title = "A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies",

abstract = "A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.",

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author = "Sofija Andjelic and Xhevat Lumi and Zolt{\'a}n Ver{\'e}b and Natasha Josifovska and Andrea Facsk{\'o} and Marko Hawlina and Goran Petrovski",

year = "2014",

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doi = "10.1155/2014/232659",

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T1 - A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

AU - Andjelic, Sofija

AU - Lumi, Xhevat

AU - Veréb, Zoltán

AU - Josifovska, Natasha

AU - Facskó, Andrea

AU - Hawlina, Marko

AU - Petrovski, Goran

PY - 2014/9/4

Y1 - 2014/9/4

N2 - A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

AB - A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

KW - viscoelastic

KW - anterior lens capsule

KW - lens epithelial cells

KW - fibrovascular epiretinal membranes

KW - proliferative diabetic retinopathy

KW - calcium imaging

KW - acetylcholine

KW - mechanostimulation

KW - inflammation

KW - IL-6

KW - IL-8

KW - TNFa

U2 - 10.1155/2014/232659

DO - 10.1155/2014/232659

M3 - Article

SN - 2314-7156

VL - 2014

SP - 1

EP - 10

JO - Journal of immunology research

JF - Journal of immunology research

ER -

A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Abstract

Keywords

UN SDGs

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