A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Sofija Andjelic, Xhevat Lumi, Zoltán Veréb, Natasha Josifovska, Andrea Facskó, Marko Hawlina, Goran Petrovski

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.
    LanguageEnglish
    Pages1-10
    JournalJournal of immunology research
    Volume2014
    DOIs
    Publication statusPublished - 4 Sep 2014

    Fingerprint

    Anterior Capsule of the Lens
    Lenses
    Epiretinal Membrane
    Epithelial Cells
    Pathology
    Calcium
    Acetylcholine
    Calcium Signaling
    Cholinergic Receptors
    Diabetic Retinopathy
    Cataract
    Cultured Cells

    Keywords

    • viscoelastic
    • anterior lens capsule
    • lens epithelial cells
    • fibrovascular epiretinal membranes
    • proliferative diabetic retinopathy
    • calcium imaging
    • acetylcholine
    • mechanostimulation
    • inflammation
    • IL-6
    • IL-8
    • TNFa

    Cite this

    Andjelic, Sofija ; Lumi, Xhevat ; Veréb, Zoltán ; Josifovska, Natasha ; Facskó, Andrea ; Hawlina, Marko ; Petrovski, Goran. / A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. In: Journal of immunology research. 2014 ; Vol. 2014. pp. 1-10.
    @article{3ef24cc7bacd447b87008565146aac56,
    title = "A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies",
    abstract = "A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.",
    keywords = "viscoelastic, anterior lens capsule, lens epithelial cells, fibrovascular epiretinal membranes, proliferative diabetic retinopathy, calcium imaging, acetylcholine, mechanostimulation, inflammation, IL-6, IL-8, TNFa",
    author = "Sofija Andjelic and Xhevat Lumi and Zolt{\'a}n Ver{\'e}b and Natasha Josifovska and Andrea Facsk{\'o} and Marko Hawlina and Goran Petrovski",
    note = "Reference text: 1. Higashide T, Sugiyama K. Use of viscoelastic substance in ophthalmic surgery—focus on sodium hyaluronate. Journal of Clinical Ophthalmology. 2008;2(1):21–30. [PMC free article] [PubMed] 2. Koskela UE, Kuusisto SM, Nissinen AE, Savolainen MJ, Liinamaa MJ. High vitreous concentration of IL-6 and IL-8, but not of adhesion molecules in relation to plasma concentrations in proliferative diabetic retinopathy. Ophthalmic Research. 2013;49(2):108–114. [PubMed] 3. Zhou J, Wang S, Xia X. Role of intravitreal inflammatory cytokines and angiogenic factors in proliferative diabetic retinopathy. Current Eye Research. 2012;37(5):416–420. [PubMed] 4. Clapham DE. Calcium Signaling. Cell. 2007;131(6):1047–1058. [PubMed] 5. Vereb Z, Lumi X, Andjelic S, et al. Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy. BioMed Research International. 2013;2013:14 pages.492376 [PMC free article] [PubMed] 6. Liu L, Paterson CA, Borchman D. Regulation of sarco/endoplasmic Ca2+-ATPase expression by calcium in human lens cells. Experimental Eye Research. 2002;75(5):583–590. [PubMed] 7. Duncan G, Wormstone IM. Calcium cell signalling and cataract: role of the endoplasmic reticulum. Eye. 1999;13, part 3b:480–483. [PubMed] 8. Churchill GC, Lurtz MM, Louis CF. Ca2+ regulation of gap junctional coupling in lens epithelial cells. American Journal of Physiology—Cell Physiology. 2001;281(3):C972–C981. [PubMed] 9. Yawata K, Nagata M, Narita A, Kawai Y. Effects of long-term acidification of extracellular pH on ATP-induced calcium mobilization in rabbit lens epithelial cells. Japanese Journal of Physiology. 2001;51(1):81–87. [PubMed] 10. Churchill GC, Atkinson MM, Louis CF. Mechanical stimulation initiates cell-to-cell calcium signaling in ovine lens epithelial cells. Journal of Cell Science. 1996;109, part 2:355–365. [PubMed] 11. Andjelić S, Zupančič G, Perovšek D, Hawlina M. Human anterior lens capsule epithelial cells contraction. Acta Ophthalmologica. 2011;89(8):e645–e653. [PubMed] 12. Collison DJ, Coleman RA, James RS, Carey J, Duncan G. Characterization of muscarinic receptors in human lens cells by pharmacologic and molecular techniques. Investigative Ophthalmology and Visual Science. 2000;41(9):2633–2641. [PubMed] 13. Collison DJ, Duncan G. Regional differences in functional receptor distribution and calcium mobilization in the intact human lens. Investigative Ophthalmology and Visual Science. 2001;42(10):2355–2363. [PubMed] 14. Rhodes JD, Sanderson J. The mechanisms of calcium homeostasis and signalling in the lens. Experimental Eye Research. 2009;88(2):226–234. [PubMed] 15. Horiuchi Y, Kimura R, Kato N, et al. Evolutional study on acetylcholine expression. Life Sciences. 2003;72(15):1745–1756. [PubMed] 16. Fujii T, Kawashima K. An independent non-neuronal cholinergic system in lymphocytes. Japanese Journal of Pharmacology. 2001;85(1):11–15. [PubMed] 17. Kawashima K, Fujii T. The lymphocytic cholinergic system and its contribution to the regulation of immune activity. Life Sciences. 2003;74(6):675–696. [PubMed] 18. Kawashima K, Fujii T, Moriwaki Y, Misawa H, Horiguchi K. Reconciling neuronally and nonneuronally derived acetylcholine in the regulation of immune function. Annals of the New York Academy of Sciences. 2012;1261(1):7–17. [PubMed] 19. Reardon C, Duncan GS, Br{\"u}stle A, et al. Lymphocyte-derived ACh regulates local innate but not adaptive immunity. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(4):1410–1415. [PMC free article] [PubMed] 20. Neumann S, Razen M, Habermehl P, et al. The non-neuronal cholinergic system in peripheral blood cells: effects of nicotinic and muscarinic receptor antagonists on phagocytosis, respiratory burst and migration. Life Sciences. 2007;80(24-25):2361–2364. [PubMed] 21. Okuma Y, Nomura Y. Roles of muscarinic acetylcholine receptors in interleukin-2 synthesis in lymphocytes. Japanese Journal of Pharmacology. 2001;85(1):16–19. [PubMed] 22. Matsumoto H, Shibasaki K, Uchigashima M, et al. Localization of acetylcholine-related molecules in the retina: Implication of the communication from photoreceptor to retinal pigment epithelium. PLoS ONE. 2012;7(8)e42841 [PMC free article] [PubMed] 23. Maneu V, Gerona G, Fern{\'a}ndez L, Cuenca N, Lax P. Evidence of alpha 7 nicotinic acetylcholine receptor expression in retinal pigment epithelial cells. Visual Neuroscience. 2010;27(5-6):139–147. [PubMed] 24. Osborne-Hereford AV, Rogers SW, Gahring LC. Neuronal nicotinic alpha7 receptors modulate inflammatory cytokine production in the skin following ultraviolet radiation. Journal of Neuroimmunology. 2008;193(1-2):130–139. [PMC free article] [PubMed] 25. Pladzyk A, Reddy ABM, Yadav UCS, Tammali R, Ramana KV, Srivastava SK. Inhibition of aldose reductase prevents lipopolysaccharide-induced inflammatory response in human lens epithelial cells. Investigative Ophthalmology and Visual Science. 2006;47(12):5395–5403. [PubMed] 26. Blatteis CM, Li S, Li Z, Feleder C, Perlik V. Cytokines, PGE2 and endotoxic fever: a re-assessment. Prostaglandins and Other Lipid Mediators. 2005;76(1–4):1–18. [PubMed] 27. Ivanov AI, Romanovsky AA. Prostaglandin E2 as a mediator of fever: synthesis and catabolism. Frontiers in Bioscience. 2004;9:1977–1993. [PubMed] 28. de Angelo J. Nitric oxide scavengers in the treatment of shock associated with systemic inflammatory response syndrome. Expert Opinion on Pharmacotherapy. 1999;1(1):19–29. [PubMed] 29. Penland RL, Boniuk M, Wilhelmus KR. Vibrio ocular infections on the U.S. Gulf Coast. Cornea. 2000;19(1):26–29. [PubMed] 30. Hazlett LD. Corneal response to Pseudomonas aeruginosa infection. Progress in Retinal and Eye Research. 2004;23(1):1–30. [PubMed] 31. Sack RA, Nunes I, Beaton A, Morris C. Host-defense mechanism of the ocular surfaces. Bioscience Reports. 2001;21(4):463–480. [PubMed] 32. Palexas GN, Sussman G, Welsh NH. Ocular and systemic determination of IL-1β and tumour necrosis factor in a patient with ocular inflammation. Scandinavian Journal of Immunology. 1992;36:173–175. [PubMed] 33. Nishi K, Nishi O, Omoto Y. The synthesis of cytokines by human lens epithelial cells—interleukin 1 (IL-1), tumor necrosis factor (TNF) interleukin 6 (IL-6), and epidermal growth factor (EGF) Nihon Ganka Gakkai Zasshi. 1992;96(6):715–720. [PubMed] 34. Sachdev NH, Di Girolamo N, Nolan TM, McCluskey PJ, Wakefield D, Coroneo MT. Matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in the human lens: implications for cortical cataract formation. Investigative Ophthalmology & Visual Science. 2004;45(11):4075–4082. [PubMed] 35. Prada J, Ngo-Tu T, Baatz H, Hartmann C, Pleyer U. Detection of tumor necrosis factor alpha and interleukin 1 alpha gene expression in human lens epithelial cells. Journal of Cataract and Refractive Surgery. 2000;26(1):114–117. [PubMed] 36. Ramana KV, Friedrich B, Bhatnagar A, Srivastava SK. Aldose reductase mediates cytotoxic signals of hyperglycemia and TNF-alpha in human lens epithelial cells. The FASEB Journal. 2003;17(2):315–317. [PubMed] 37. Collins T, Cybulsky MI. NF-κB: pivotal mediator or innocent bystander in atherogenesis? The Journal of Clinical Investigation. 2001;107(3):255–264. [PMC free article] [PubMed] 38. Nishi O, Nishi K, Wada K, Ohmoto Y. Expression of transforming growth factor (TGF)-α, TGF-β2 and interleukin 8 messenger RNA in postsurgical and cultured lens epithelial cells obtained from patients with senile cataracts. Graefe's Archive for Clinical and Experimental Ophthalmology. 1999;237(10):806–811. [PubMed] 39. Nishi O, Nishi K, Imanishi M, Tada Y, Shirasawa E. Effect of the cytokines on the prostaglandin E2 synthesis by lens epithelial cells of human cataracts. British Journal of Ophthalmology. 1995;79(10):934–938. [PMC free article] [PubMed] 40. Nishi O, Nishi K, Ohmoto Y. Synthesis of interleukin-1, interleukin-6, and basic fibroblast growth factor by human cataract lens epithelial cells. Journal of Cataract & Refractive Surgery. 1996;22(supplement 1):852–858. [PubMed] 41. Harocopos GJ, Alvares KM, Kolker AE, Beebe DC. Human age-related cataract and lens epithelial cell death. Investigative Ophthalmology & Visual Science. 1998;39(13):2696–2706. [PubMed] 42. Limb GA, Chignell AH, Green W, LeRoy F, Dumonde DC. Distribution of TNFα and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy. British Journal of Ophthalmology. 1996;80(2):168–173. [PMC free article] [PubMed] 43. Gustavsson C, Agardh E, Bengtsson B, Agardh C-D. TNF-α is an independent serum marker for proliferative retinopathy in type 1 diabetic patients. Journal of Diabetes and its Complications. 2008;22(5):309–316. [PubMed] 44. Limb GA, Cole CJ, Earley O, Hollifield RD, Russell W, Stanford MR. Expression of hematopoietic cell markers by retinal pigment epithelial cells. Current Eye Research. 1997;16(10):985–991. [PubMed] 45. Platts KF, Benson MT, Rennie IG, Sharrard RM, Rees RC. Cytokine modulation of adhesion molecule expression on human retinal pigment epithelial cells. Investigative Ophthalmology and Visual Science. 1995;36(11):2262–2269. [PubMed] 46. Elner SG, Elner VM, Pavilack MA, et al. Modulation and function of intercellular adhesion molecule-1 (CD54) on human retinal pigment epithelial cells. Laboratory Investigation. 1992;66(2):200–211. [PubMed] 47. Kanuga N, Winton HL, Beauch{\'e}ne L, et al. Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies. Investigative Ophthalmology and Visual Science. 2002;43(2):546–555. [PubMed] 48. Kumar MV, Nagineni CN, Chin MS, Hooks JJ, Detrick B. Innate immunity in the retina: toll-like receptor (TLR) signaling in human retinal pigment epithelial cells. Journal of Neuroimmunology. 2004;153(1-2):7–15. [PubMed] 49. Heidenkummer H-P, Kampik A. Intercellular adhesion molecule-1 (ICAM-1) and leukocyte function-associated antigen-1 (LFA-1) expression in human epiretinal membranes. Graefe's Archive for Clinical and Experimental Ophthalmology. 1992;230(5):483–487. [PubMed] 50. Devine L, Lightman SL, Greenwood J. Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology. 1996;88(3):456–462. [PMC free article] [PubMed] 51. Patel JI, Saleh GM, Hykin PG, Gregor ZJ, Cree IA. Concentration of haemodynamic and inflammatory related cytokines in diabetic retinopathy. Eye. 2008;22(2):223–228. [PubMed] 52. K{\"o}hl J. Anaphylatoxins and infectious and non-infectious inflammatory diseases. Molecular Immunology. 2001;38(2-3):175–187. [PubMed] 53. Van Beijnum JR, Buurman WA, Griffioen AW. Convergence and amplification of toll-like receptor (TLR) and receptor for advanced glycation end products (RAGE) signaling pathways via high mobility group B1 (HMGB1) Angiogenesis. 2008;11(1):91–99. [PubMed] 54. Treutiger CJ, Mullins GE, Johansson A-SM, et al. High mobility group 1 B-box mediates activation of human endothelium. Journal of Internal Medicine. 2003;254(4):375–385. [PubMed] 55. Fiuza C, Bustin M, Talwar S, et al. Inflammation-promoting activity of HMGB1 on human microvascular endothelial cells. Blood. 2003;101(7):2652–2660. [PubMed] 56. Luan Z-G, Zhang H, Yang P-T, Ma X-C, Zhang C, Guo R-X. HMGB1 activates nuclear factor-κB signaling by RAGE and increases the production of TNF-α in human umbilical vein endothelial cells. Immunobiology. 2010;215(12):956–962. [PubMed] 57. Mitola S, Belleri M, Urbinati C, et al. Cutting edge: extracellular high mobility group box-1 protein is a proangiogenic cytokine. Journal of Immunology. 2006;176(1):12–15. [PubMed] 58. Schlueter C, Weber H, Meyer B, et al. Angiogenetic signaling through hypoxia HMGB1: an angiogenetic switch molecule. The American Journal of Pathology. 2005;166(4):1259–1263. [PMC free article] [PubMed] 59. Chavakis E, Hain A, Vinci M, et al. High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells. Circulation Research. 2007;100(2):204–212. [PubMed] 60. El-Asrar AMA, Nawaz MI, Kangave D, et al. High-mobility group box-1 and biomarkers of inflammation in the vitreous from patients with proliferative diabetic retinopathy. Molecular Vision. 2011;17:1829–1838. [PMC free article] [PubMed] 61. Bromberg-White JL, Glazer L, Downer R, et al. Identification of VEGF-independent cytokines in proliferative diabetic retinopathy vitreous. Investigative Ophthalmology & Visual Science. 2013;54(10):6472–6480. [PubMed]",
    year = "2014",
    month = "9",
    day = "4",
    doi = "10.1155/2014/232659",
    language = "English",
    volume = "2014",
    pages = "1--10",
    journal = "Journal of immunology research",
    issn = "2314-8861",

    }

    A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies. / Andjelic, Sofija; Lumi, Xhevat; Veréb, Zoltán; Josifovska, Natasha; Facskó, Andrea; Hawlina, Marko; Petrovski, Goran.

    In: Journal of immunology research, Vol. 2014, 04.09.2014, p. 1-10.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

    AU - Andjelic, Sofija

    AU - Lumi, Xhevat

    AU - Veréb, Zoltán

    AU - Josifovska, Natasha

    AU - Facskó, Andrea

    AU - Hawlina, Marko

    AU - Petrovski, Goran

    N1 - Reference text: 1. Higashide T, Sugiyama K. Use of viscoelastic substance in ophthalmic surgery—focus on sodium hyaluronate. Journal of Clinical Ophthalmology. 2008;2(1):21–30. [PMC free article] [PubMed] 2. Koskela UE, Kuusisto SM, Nissinen AE, Savolainen MJ, Liinamaa MJ. High vitreous concentration of IL-6 and IL-8, but not of adhesion molecules in relation to plasma concentrations in proliferative diabetic retinopathy. Ophthalmic Research. 2013;49(2):108–114. [PubMed] 3. Zhou J, Wang S, Xia X. Role of intravitreal inflammatory cytokines and angiogenic factors in proliferative diabetic retinopathy. Current Eye Research. 2012;37(5):416–420. [PubMed] 4. Clapham DE. Calcium Signaling. Cell. 2007;131(6):1047–1058. [PubMed] 5. Vereb Z, Lumi X, Andjelic S, et al. Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy. BioMed Research International. 2013;2013:14 pages.492376 [PMC free article] [PubMed] 6. Liu L, Paterson CA, Borchman D. Regulation of sarco/endoplasmic Ca2+-ATPase expression by calcium in human lens cells. Experimental Eye Research. 2002;75(5):583–590. [PubMed] 7. Duncan G, Wormstone IM. Calcium cell signalling and cataract: role of the endoplasmic reticulum. Eye. 1999;13, part 3b:480–483. [PubMed] 8. Churchill GC, Lurtz MM, Louis CF. Ca2+ regulation of gap junctional coupling in lens epithelial cells. American Journal of Physiology—Cell Physiology. 2001;281(3):C972–C981. [PubMed] 9. Yawata K, Nagata M, Narita A, Kawai Y. Effects of long-term acidification of extracellular pH on ATP-induced calcium mobilization in rabbit lens epithelial cells. Japanese Journal of Physiology. 2001;51(1):81–87. [PubMed] 10. Churchill GC, Atkinson MM, Louis CF. Mechanical stimulation initiates cell-to-cell calcium signaling in ovine lens epithelial cells. Journal of Cell Science. 1996;109, part 2:355–365. [PubMed] 11. Andjelić S, Zupančič G, Perovšek D, Hawlina M. Human anterior lens capsule epithelial cells contraction. Acta Ophthalmologica. 2011;89(8):e645–e653. [PubMed] 12. Collison DJ, Coleman RA, James RS, Carey J, Duncan G. Characterization of muscarinic receptors in human lens cells by pharmacologic and molecular techniques. Investigative Ophthalmology and Visual Science. 2000;41(9):2633–2641. [PubMed] 13. Collison DJ, Duncan G. Regional differences in functional receptor distribution and calcium mobilization in the intact human lens. Investigative Ophthalmology and Visual Science. 2001;42(10):2355–2363. [PubMed] 14. Rhodes JD, Sanderson J. The mechanisms of calcium homeostasis and signalling in the lens. Experimental Eye Research. 2009;88(2):226–234. [PubMed] 15. Horiuchi Y, Kimura R, Kato N, et al. Evolutional study on acetylcholine expression. Life Sciences. 2003;72(15):1745–1756. [PubMed] 16. Fujii T, Kawashima K. An independent non-neuronal cholinergic system in lymphocytes. Japanese Journal of Pharmacology. 2001;85(1):11–15. [PubMed] 17. Kawashima K, Fujii T. The lymphocytic cholinergic system and its contribution to the regulation of immune activity. Life Sciences. 2003;74(6):675–696. [PubMed] 18. Kawashima K, Fujii T, Moriwaki Y, Misawa H, Horiguchi K. Reconciling neuronally and nonneuronally derived acetylcholine in the regulation of immune function. Annals of the New York Academy of Sciences. 2012;1261(1):7–17. [PubMed] 19. Reardon C, Duncan GS, Brüstle A, et al. Lymphocyte-derived ACh regulates local innate but not adaptive immunity. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(4):1410–1415. [PMC free article] [PubMed] 20. Neumann S, Razen M, Habermehl P, et al. The non-neuronal cholinergic system in peripheral blood cells: effects of nicotinic and muscarinic receptor antagonists on phagocytosis, respiratory burst and migration. Life Sciences. 2007;80(24-25):2361–2364. [PubMed] 21. Okuma Y, Nomura Y. Roles of muscarinic acetylcholine receptors in interleukin-2 synthesis in lymphocytes. Japanese Journal of Pharmacology. 2001;85(1):16–19. [PubMed] 22. Matsumoto H, Shibasaki K, Uchigashima M, et al. Localization of acetylcholine-related molecules in the retina: Implication of the communication from photoreceptor to retinal pigment epithelium. PLoS ONE. 2012;7(8)e42841 [PMC free article] [PubMed] 23. Maneu V, Gerona G, Fernández L, Cuenca N, Lax P. Evidence of alpha 7 nicotinic acetylcholine receptor expression in retinal pigment epithelial cells. Visual Neuroscience. 2010;27(5-6):139–147. [PubMed] 24. Osborne-Hereford AV, Rogers SW, Gahring LC. Neuronal nicotinic alpha7 receptors modulate inflammatory cytokine production in the skin following ultraviolet radiation. Journal of Neuroimmunology. 2008;193(1-2):130–139. [PMC free article] [PubMed] 25. Pladzyk A, Reddy ABM, Yadav UCS, Tammali R, Ramana KV, Srivastava SK. Inhibition of aldose reductase prevents lipopolysaccharide-induced inflammatory response in human lens epithelial cells. Investigative Ophthalmology and Visual Science. 2006;47(12):5395–5403. [PubMed] 26. Blatteis CM, Li S, Li Z, Feleder C, Perlik V. Cytokines, PGE2 and endotoxic fever: a re-assessment. Prostaglandins and Other Lipid Mediators. 2005;76(1–4):1–18. [PubMed] 27. Ivanov AI, Romanovsky AA. Prostaglandin E2 as a mediator of fever: synthesis and catabolism. Frontiers in Bioscience. 2004;9:1977–1993. [PubMed] 28. de Angelo J. Nitric oxide scavengers in the treatment of shock associated with systemic inflammatory response syndrome. Expert Opinion on Pharmacotherapy. 1999;1(1):19–29. [PubMed] 29. Penland RL, Boniuk M, Wilhelmus KR. Vibrio ocular infections on the U.S. Gulf Coast. Cornea. 2000;19(1):26–29. [PubMed] 30. Hazlett LD. Corneal response to Pseudomonas aeruginosa infection. Progress in Retinal and Eye Research. 2004;23(1):1–30. [PubMed] 31. Sack RA, Nunes I, Beaton A, Morris C. Host-defense mechanism of the ocular surfaces. Bioscience Reports. 2001;21(4):463–480. [PubMed] 32. Palexas GN, Sussman G, Welsh NH. Ocular and systemic determination of IL-1β and tumour necrosis factor in a patient with ocular inflammation. Scandinavian Journal of Immunology. 1992;36:173–175. [PubMed] 33. Nishi K, Nishi O, Omoto Y. The synthesis of cytokines by human lens epithelial cells—interleukin 1 (IL-1), tumor necrosis factor (TNF) interleukin 6 (IL-6), and epidermal growth factor (EGF) Nihon Ganka Gakkai Zasshi. 1992;96(6):715–720. [PubMed] 34. Sachdev NH, Di Girolamo N, Nolan TM, McCluskey PJ, Wakefield D, Coroneo MT. Matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in the human lens: implications for cortical cataract formation. Investigative Ophthalmology & Visual Science. 2004;45(11):4075–4082. [PubMed] 35. Prada J, Ngo-Tu T, Baatz H, Hartmann C, Pleyer U. Detection of tumor necrosis factor alpha and interleukin 1 alpha gene expression in human lens epithelial cells. Journal of Cataract and Refractive Surgery. 2000;26(1):114–117. [PubMed] 36. Ramana KV, Friedrich B, Bhatnagar A, Srivastava SK. Aldose reductase mediates cytotoxic signals of hyperglycemia and TNF-alpha in human lens epithelial cells. The FASEB Journal. 2003;17(2):315–317. [PubMed] 37. Collins T, Cybulsky MI. NF-κB: pivotal mediator or innocent bystander in atherogenesis? The Journal of Clinical Investigation. 2001;107(3):255–264. [PMC free article] [PubMed] 38. Nishi O, Nishi K, Wada K, Ohmoto Y. Expression of transforming growth factor (TGF)-α, TGF-β2 and interleukin 8 messenger RNA in postsurgical and cultured lens epithelial cells obtained from patients with senile cataracts. Graefe's Archive for Clinical and Experimental Ophthalmology. 1999;237(10):806–811. [PubMed] 39. Nishi O, Nishi K, Imanishi M, Tada Y, Shirasawa E. Effect of the cytokines on the prostaglandin E2 synthesis by lens epithelial cells of human cataracts. British Journal of Ophthalmology. 1995;79(10):934–938. [PMC free article] [PubMed] 40. Nishi O, Nishi K, Ohmoto Y. Synthesis of interleukin-1, interleukin-6, and basic fibroblast growth factor by human cataract lens epithelial cells. Journal of Cataract & Refractive Surgery. 1996;22(supplement 1):852–858. [PubMed] 41. Harocopos GJ, Alvares KM, Kolker AE, Beebe DC. Human age-related cataract and lens epithelial cell death. Investigative Ophthalmology & Visual Science. 1998;39(13):2696–2706. [PubMed] 42. Limb GA, Chignell AH, Green W, LeRoy F, Dumonde DC. Distribution of TNFα and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy. British Journal of Ophthalmology. 1996;80(2):168–173. [PMC free article] [PubMed] 43. Gustavsson C, Agardh E, Bengtsson B, Agardh C-D. TNF-α is an independent serum marker for proliferative retinopathy in type 1 diabetic patients. Journal of Diabetes and its Complications. 2008;22(5):309–316. [PubMed] 44. Limb GA, Cole CJ, Earley O, Hollifield RD, Russell W, Stanford MR. Expression of hematopoietic cell markers by retinal pigment epithelial cells. Current Eye Research. 1997;16(10):985–991. [PubMed] 45. Platts KF, Benson MT, Rennie IG, Sharrard RM, Rees RC. Cytokine modulation of adhesion molecule expression on human retinal pigment epithelial cells. Investigative Ophthalmology and Visual Science. 1995;36(11):2262–2269. [PubMed] 46. Elner SG, Elner VM, Pavilack MA, et al. Modulation and function of intercellular adhesion molecule-1 (CD54) on human retinal pigment epithelial cells. Laboratory Investigation. 1992;66(2):200–211. [PubMed] 47. Kanuga N, Winton HL, Beauchéne L, et al. Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies. Investigative Ophthalmology and Visual Science. 2002;43(2):546–555. [PubMed] 48. Kumar MV, Nagineni CN, Chin MS, Hooks JJ, Detrick B. Innate immunity in the retina: toll-like receptor (TLR) signaling in human retinal pigment epithelial cells. Journal of Neuroimmunology. 2004;153(1-2):7–15. [PubMed] 49. Heidenkummer H-P, Kampik A. Intercellular adhesion molecule-1 (ICAM-1) and leukocyte function-associated antigen-1 (LFA-1) expression in human epiretinal membranes. Graefe's Archive for Clinical and Experimental Ophthalmology. 1992;230(5):483–487. [PubMed] 50. Devine L, Lightman SL, Greenwood J. Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology. 1996;88(3):456–462. [PMC free article] [PubMed] 51. Patel JI, Saleh GM, Hykin PG, Gregor ZJ, Cree IA. Concentration of haemodynamic and inflammatory related cytokines in diabetic retinopathy. Eye. 2008;22(2):223–228. [PubMed] 52. Köhl J. 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    PY - 2014/9/4

    Y1 - 2014/9/4

    N2 - A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

    AB - A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.

    KW - viscoelastic

    KW - anterior lens capsule

    KW - lens epithelial cells

    KW - fibrovascular epiretinal membranes

    KW - proliferative diabetic retinopathy

    KW - calcium imaging

    KW - acetylcholine

    KW - mechanostimulation

    KW - inflammation

    KW - IL-6

    KW - IL-8

    KW - TNFa

    U2 - 10.1155/2014/232659

    DO - 10.1155/2014/232659

    M3 - Article

    VL - 2014

    SP - 1

    EP - 10

    JO - Journal of immunology research

    T2 - Journal of immunology research

    JF - Journal of immunology research

    SN - 2314-8861

    ER -