A Simple Method for Establishing AdherentEx VivoExplant Cultures from Human Eye Pathologies for Use in Subsequent Calcium Imaging and Inflammatory Studies

Sofija Andjelic, Xhevat Lumi, Zoltán Veréb, Natasha Josifovska, Andrea Facskó, Marko Hawlina, Goran Petrovski

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    A novel, simple, and reproducible method for cultivating pathological tissues obtained from human eyes during surgery was developed using viscoelastic material as a tissue adherent to facilitate cell attachment and expansion and calcium imaging of cultured cells challenged by mechanical and acetylcholine (ACh) stimulation as well as inflammatory studies. Anterior lens capsule-lens epithelial cells (aLC-LECs) from cataract surgery and proliferative diabetic retinopathy (PDR) fibrovascular epiretinal membranes (fvERMs) from human eyes were used in the study. We hereby show calcium signaling in aLC-LECs by mechanical and acetylcholine (ACh) stimulation and indicate presence of ACh receptors in these cells. Furthermore, an ex vivo study model was established for measuring the inflammatory response in fvERMs and aLC-LECs upon TNFα treatment.
    Original languageEnglish
    Pages (from-to)1-10
    JournalJournal of immunology research
    Volume2014
    DOIs
    Publication statusPublished - 4 Sep 2014

    Keywords

    • viscoelastic
    • anterior lens capsule
    • lens epithelial cells
    • fibrovascular epiretinal membranes
    • proliferative diabetic retinopathy
    • calcium imaging
    • acetylcholine
    • mechanostimulation
    • inflammation
    • IL-6
    • IL-8
    • TNFa

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