A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources

RV Muralidhar, RR Chirumamila, R Marchant, Poonam Singh - Nee Nigam

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Abstract

A response surface approach has been used to study the production of extracellular lipase by Candida cylindracea. Medium constituents were optimised for the production of lipase by central composite design. A 2(5) design matrix was used in the experiments with only the carbon source being varied. The optimal yield of lipase was 17.30 U/ml using glucose as the carbon source and 47.25 U/ml using olive oil as the carbon source. All the other medium constituents namely yeast extract, malt extract, peptone and tween 80 were simultaneously optimised. Experiments conducted at the predicted optimum levels fitted with the predicted optima. Based on these experimental conditions, medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase. (C) 2001 Elsevier Science B.V. All rights reserved.
LanguageEnglish
Pages17-23
JournalBiochemical Engineering Journal
Volume9
Issue number1
Publication statusPublished - Nov 2001

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carbon
oil
yeast
glucose
experiment
matrix
comparison

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abstract = "A response surface approach has been used to study the production of extracellular lipase by Candida cylindracea. Medium constituents were optimised for the production of lipase by central composite design. A 2(5) design matrix was used in the experiments with only the carbon source being varied. The optimal yield of lipase was 17.30 U/ml using glucose as the carbon source and 47.25 U/ml using olive oil as the carbon source. All the other medium constituents namely yeast extract, malt extract, peptone and tween 80 were simultaneously optimised. Experiments conducted at the predicted optimum levels fitted with the predicted optima. Based on these experimental conditions, medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase. (C) 2001 Elsevier Science B.V. All rights reserved.",
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AU - Marchant, R

AU - Singh - Nee Nigam, Poonam

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AB - A response surface approach has been used to study the production of extracellular lipase by Candida cylindracea. Medium constituents were optimised for the production of lipase by central composite design. A 2(5) design matrix was used in the experiments with only the carbon source being varied. The optimal yield of lipase was 17.30 U/ml using glucose as the carbon source and 47.25 U/ml using olive oil as the carbon source. All the other medium constituents namely yeast extract, malt extract, peptone and tween 80 were simultaneously optimised. Experiments conducted at the predicted optimum levels fitted with the predicted optima. Based on these experimental conditions, medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase. (C) 2001 Elsevier Science B.V. All rights reserved.

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