Objectives: The plant virus cowpea mosaic virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty virus-like particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications.
Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid.
Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis have a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 µM respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents.
Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines, and its potency increased 2.3-folds.
|Number of pages||29|
|Journal||Combinatorial Chemistry & High Throughput Screening|
|Publication status||Published - 14 Sep 2020|