A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)

J. Michael Conlon; Gavin J. Power; Yasser Abdel-Wahab; Peter Flatt; Hu Jiansheng; Laurent Coquet; Jerome Leprince; Thierry Jouenne; Hubert Vaudry

doi:10.1016/j.regpep.2008.04.002

A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)

J. Michael Conlon, Gavin J. Power, Yasser Abdel-Wahab, Peter Flatt, Hu Jiansheng, Laurent Coquet, Jerome Leprince, Thierry Jouenne, Hubert Vaudry

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Abstract

Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P

Original language	English
Pages (from-to)	153-159
Journal	Regulatory Peptides
Volume	151
Issue number	1-3
DOIs	https://doi.org/10.1016/j.regpep.2008.04.002
Publication status	Published (in print/issue) - Nov 2008

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@article{29971b66a287429cb54c7fa76b53b1d1,

title = "A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)",

abstract = "Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P",

author = "Conlon, {J. Michael} and Power, {Gavin J.} and Yasser Abdel-Wahab and Peter Flatt and Hu Jiansheng and Laurent Coquet and Jerome Leprince and Thierry Jouenne and Hubert Vaudry",

year = "2008",

month = nov,

doi = "10.1016/j.regpep.2008.04.002",

language = "English",

volume = "151",

pages = "153--159",

journal = "Regulatory Peptides",

issn = "0167-0115",

publisher = "Elsevier",

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T1 - A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)

AU - Conlon, J. Michael

AU - Power, Gavin J.

AU - Abdel-Wahab, Yasser

AU - Flatt, Peter

AU - Jiansheng, Hu

AU - Coquet, Laurent

AU - Leprince, Jerome

AU - Jouenne, Thierry

AU - Vaudry, Hubert

PY - 2008/11

Y1 - 2008/11

N2 - Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P

AB - Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P

U2 - 10.1016/j.regpep.2008.04.002

DO - 10.1016/j.regpep.2008.04.002

M3 - Article

VL - 151

SP - 153

EP - 159

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-3

ER -

A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)

Abstract

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