A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)

J. Michael Conlon, Gavin J. Power, Yasser Abdel-Wahab, Peter Flatt, Hu Jiansheng, Laurent Coquet, Jerome Leprince, Thierry Jouenne, Hubert Vaudry

Research output: Contribution to journal › Article

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Abstract

Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P
LanguageEnglish
Pages153-159
JournalRegulatory Peptides
Volume151
Issue number1-3
DOIs
Publication statusPublished - Nov 2008

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Ranidae
Anura
Insulin
Skin
Peptides
L-Lactate Dehydrogenase
High Pressure Liquid Chromatography
Cell Line
Enzymes

Cite this

Conlon, J. Michael ; Power, Gavin J. ; Abdel-Wahab, Yasser ; Flatt, Peter ; Jiansheng, Hu ; Coquet, Laurent ; Leprince, Jerome ; Jouenne, Thierry ; Vaudry, Hubert. / A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae). In: Regulatory Peptides. 2008 ; Vol. 151, No. 1-3. pp. 153-159.
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abstract = "Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139{\%} of basal rate; P",
author = "Conlon, {J. Michael} and Power, {Gavin J.} and Yasser Abdel-Wahab and Peter Flatt and Hu Jiansheng and Laurent Coquet and Jerome Leprince and Thierry Jouenne and Hubert Vaudry",
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Conlon, JM, Power, GJ, Abdel-Wahab, Y, Flatt, P, Jiansheng, H, Coquet, L, Leprince, J, Jouenne, T & Vaudry, H 2008, 'A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)', Regulatory Peptides, vol. 151, no. 1-3, pp. 153-159. https://doi.org/10.1016/j.regpep.2008.04.002

A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae). / Conlon, J. Michael; Power, Gavin J.; Abdel-Wahab, Yasser; Flatt, Peter; Jiansheng, Hu; Coquet, Laurent; Leprince, Jerome; Jouenne, Thierry; Vaudry, Hubert.

In: Regulatory Peptides, Vol. 151, No. 1-3, 11.2008, p. 153-159.

Research output: Contribution to journal › Article

TY - JOUR

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AU - Conlon, J. Michael

AU - Power, Gavin J.

AU - Abdel-Wahab, Yasser

AU - Flatt, Peter

AU - Jiansheng, Hu

AU - Coquet, Laurent

AU - Leprince, Jerome

AU - Jouenne, Thierry

AU - Vaudry, Hubert

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AB - Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P

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Conlon JM, Power GJ, Abdel-Wahab Y, Flatt P, Jiansheng H, Coquet L et al. A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae). Regulatory Peptides. 2008 Nov;151(1-3):153-159. https://doi.org/10.1016/j.regpep.2008.04.002

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