Abstract
Language | English |
---|---|
Pages | 153-159 |
Journal | Regulatory Peptides |
Volume | 151 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Nov 2008 |
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A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae). / Conlon, J. Michael; Power, Gavin J.; Abdel-Wahab, Yasser; Flatt, Peter; Jiansheng, Hu; Coquet, Laurent; Leprince, Jerome; Jouenne, Thierry; Vaudry, Hubert.
In: Regulatory Peptides, Vol. 151, No. 1-3, 11.2008, p. 153-159.Research output: Contribution to journal › Article
TY - JOUR
T1 - A potent, non-toxic insulin-releasing peptide isolated from an extract of the skin of the Asian frog, Hylarana guntheri (Anura:Ranidae)
AU - Conlon, J. Michael
AU - Power, Gavin J.
AU - Abdel-Wahab, Yasser
AU - Flatt, Peter
AU - Jiansheng, Hu
AU - Coquet, Laurent
AU - Leprince, Jerome
AU - Jouenne, Thierry
AU - Vaudry, Hubert
PY - 2008/11
Y1 - 2008/11
N2 - Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P
AB - Peptides in extract of the skin of the Asian frog Hylarana guntheri Boulenger,1882 were purified by reversed-phase HPLC and individual components analysed for their ability to release insulin from the rat BRIN-BD11 clonal beta cell line. The most potent peptide identified in the extract belonged to the brevinin-2 family (brevinin-2GUb; GVIIDTLKGAAKTVAAELLRKAHCKLTNSC). Other peptides with weaker insulin-releasing activity belonged to the brevinin-1 (2 peptides), brevinin-2 (2 peptides) and temporin (3 peptides) families. Only the brevinin-1 peptides showed cytolytic activity against the BRIN-BD11 cells, as demonstrated by an increased rate of release of the cytosolic enzyme, lactate dehydrogenase. A synthetic replicate of brevinin-2GUb produced a significant stimulation of insulin release (139% of basal rate; P
U2 - 10.1016/j.regpep.2008.04.002
DO - 10.1016/j.regpep.2008.04.002
M3 - Article
VL - 151
SP - 153
EP - 159
JO - Regulatory Peptides
T2 - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 1-3
ER -