Abstract
Language | English |
---|---|
Pages | 137-151 |
Journal | Growth Factors |
Volume | 16 |
Issue number | 2 |
Publication status | Published - 1998 |
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A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. / Miller, SJ; Ulleras, E; Moncrieff, CL; Walsh, Colum; Adam, GIR; Franklin, GC.
In: Growth Factors, Vol. 16, No. 2, 1998, p. 137-151.Research output: Contribution to journal › Article
TY - JOUR
T1 - A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region
AU - Miller, SJ
AU - Ulleras, E
AU - Moncrieff, CL
AU - Walsh, Colum
AU - Adam, GIR
AU - Franklin, GC
PY - 1998
Y1 - 1998
N2 - We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.
AB - We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.
M3 - Article
VL - 16
SP - 137
EP - 151
JO - Growth Factors
T2 - Growth Factors
JF - Growth Factors
SN - 0897-7194
IS - 2
ER -