A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region

SJ Miller, E Ulleras, CL Moncrieff, Colum Walsh, GIR Adam, GC Franklin

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.
LanguageEnglish
Pages137-151
JournalGrowth Factors
Volume16
Issue number2
Publication statusPublished - 1998

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Proto-Oncogene Proteins c-sis
Choriocarcinoma
Gene Expression Regulation
Genes
Breast Neoplasms

Cite this

Miller, SJ., Ulleras, E., Moncrieff, CL., Walsh, C., Adam, GIR., & Franklin, GC. (1998). A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. Growth Factors, 16(2), 137-151.
Miller, SJ ; Ulleras, E ; Moncrieff, CL ; Walsh, Colum ; Adam, GIR ; Franklin, GC. / A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. In: Growth Factors. 1998 ; Vol. 16, No. 2. pp. 137-151.
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abstract = "We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.",
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Miller, SJ, Ulleras, E, Moncrieff, CL, Walsh, C, Adam, GIR & Franklin, GC 1998, 'A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region', Growth Factors, vol. 16, no. 2, pp. 137-151.

A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. / Miller, SJ; Ulleras, E; Moncrieff, CL; Walsh, Colum; Adam, GIR; Franklin, GC.

In: Growth Factors, Vol. 16, No. 2, 1998, p. 137-151.

Research output: Contribution to journal › Article

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T1 - A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region

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AU - Ulleras, E

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AU - Adam, GIR

AU - Franklin, GC

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AB - We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.

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Miller SJ, Ulleras E, Moncrieff CL, Walsh C, Adam GIR, Franklin GC. A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. Growth Factors. 1998;16(2):137-151.