A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region

SJ Miller, E Ulleras, CL Moncrieff, Colum Walsh, GIR Adam, GC Franklin

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Abstract

We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.
Original languageEnglish
Pages (from-to)137-151
JournalGrowth Factors
Volume16
Issue number2
Publication statusPublished - 1998

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    Miller, SJ., Ulleras, E., Moncrieff, CL., Walsh, C., Adam, GIR., & Franklin, GC. (1998). A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. Growth Factors, 16(2), 137-151.