A Novel Method for Quantitative Analysis of C-Reactive Protein Lateral Flow Immunoassays Images via CMOS Sensor and Recurrent Neural Networks

Min Jing, Donal McLaughlin, Sara Mc Namee, Shasidran Raj, Brian Mac Namee, David Steele, D Finlay, James McLaughlin

Research output: Contribution to journalArticlepeer-review

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Abstract

Objective: To design and implement an easy-to-use, Point-of-Care (PoC) lateral flow immunoassays (LFA) reader and data analysis system, which provides a more in-depth quantitative analysis for LFA images than conventional approaches thereby supporting efficient decision making for potential early risk assessment of cardiovascular disease (CVD).

Methods and procedures: A novel end-to-end system was developed including a portable device with CMOS camera integrated with optimized illumination and optics to capture the LFA images produced using high-sensitivity C-Reactive Protein (hsCRP) (concentration level < 5 mg/L). The images were transmitted via WiFi to a back-end server system for image analysis and classification. Unlike common image classification approaches which are based on averaging image intensity from a region-of-interest (ROI), a novel approach was developed which considered the signal along the sample’s flow direction as a time series and, consequently, no need for ROI detection. Long Short-Term Memory (LSTM) networks were deployed for multilevel classification. The features based on Dynamic Time Warping (DTW) and histogram bin counts (HBC) were explored for classification.

Results: For the classification of hsCRP, the LSTM outperformed the traditional machine learning classifiers with or without DTW and HBC features performed the best (with mean accuracy of 94%) compared to other features. Application of the proposed method to human plasma also suggests that HBC features from LFA time series performed better than the mean from ROI and raw LFA data.

Conclusion: As a proof of concept, the results demonstrate the capability of the proposed framework for quantitative analysis of LFA images and suggest the potential for early risk assessment of CVD.
Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalIEEE Journal of Translational Engineering in Health and Medicine
Early online date23 Nov 2021
DOIs
Publication statusE-pub ahead of print - 23 Nov 2021

Keywords

  • CMOS image sensor
  • Cameras
  • Dynamic Time Warping
  • Lateral Flow Immunoassays (LFA)
  • Lighting
  • Long Short-Term Memory (LSTM)
  • Proteins
  • Risk management
  • Servers
  • Statistical analysis
  • Testing
  • high-sensitivity C-Reactive Protein

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