Abstract
Here, we introduce core-shell nanofibers based on chitosan (CS)-loaded poly (ε-caprolactone) (PCL) shell and 5-fluorouracil (5-FU)-loaded Poly(N-vinyl-2-pyrrolidone) (PVP) core for synergistic therapy of melanoma skin cancer. The yielded nanofibers exhibited an average diameter of 503 nm with high drug-encapsulating efficiency and good mechanical properties. Moreover, the burst release of 5-FU significantly inhibited melanoma skin cancer cells (B16F10 cells), and the sustained release of CS exhibited “remedying effects” on normal skin cells (L929 cells) after suffering adverse effects from 5-FU treatment. For the B16F10 cells, the early apoptosis cells increased from 0.8% to 62.2% after being treated with blended films loaded with 5-FU (2 wt%) for 24 h; for the L929 cells, the vital cells increased from 68.9% to 77.0%, and the early apoptosis of stage cells decreased from 12.3% to 10.9% after being treated with blended films with CS (8 wt%) for 24 h. In conclusion, the results introduced in this work can be a promising strategy for cancer treatment and possesses synergism potential to broaden an avenue for chemotherapeutic therapy with minimum adverse effects on normal cells.
| Language | English |
|---|---|
| Article number | 105002 |
| Journal | European Journal of Pharmaceutical Sciences |
| Volume | 137 |
| Early online date | 11 Jul 2019 |
| DOIs | |
| Publication status | Published - 1 Sep 2019 |
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Keywords
- 5-Fluorouracil
- Chitosan
- Controlled release
- Core-shell nanofiber
- Skin cancer
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A novel core-shell nanofiber drug delivery system intended for the synergistic treatment of melanoma. / Zhu, Li Fang; Zheng, Yufei; Fan, Jiannan; Yao, Yuanfa; Ahmad, Zeeshan; Chang, Ming Wei.
In: European Journal of Pharmaceutical Sciences, Vol. 137, 105002, 01.09.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - A novel core-shell nanofiber drug delivery system intended for the synergistic treatment of melanoma
AU - Zhu, Li Fang
AU - Zheng, Yufei
AU - Fan, Jiannan
AU - Yao, Yuanfa
AU - Ahmad, Zeeshan
AU - Chang, Ming Wei
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Here, we introduce core-shell nanofibers based on chitosan (CS)-loaded poly (ε-caprolactone) (PCL) shell and 5-fluorouracil (5-FU)-loaded Poly(N-vinyl-2-pyrrolidone) (PVP) core for synergistic therapy of melanoma skin cancer. The yielded nanofibers exhibited an average diameter of 503 nm with high drug-encapsulating efficiency and good mechanical properties. Moreover, the burst release of 5-FU significantly inhibited melanoma skin cancer cells (B16F10 cells), and the sustained release of CS exhibited “remedying effects” on normal skin cells (L929 cells) after suffering adverse effects from 5-FU treatment. For the B16F10 cells, the early apoptosis cells increased from 0.8% to 62.2% after being treated with blended films loaded with 5-FU (2 wt%) for 24 h; for the L929 cells, the vital cells increased from 68.9% to 77.0%, and the early apoptosis of stage cells decreased from 12.3% to 10.9% after being treated with blended films with CS (8 wt%) for 24 h. In conclusion, the results introduced in this work can be a promising strategy for cancer treatment and possesses synergism potential to broaden an avenue for chemotherapeutic therapy with minimum adverse effects on normal cells.
AB - Here, we introduce core-shell nanofibers based on chitosan (CS)-loaded poly (ε-caprolactone) (PCL) shell and 5-fluorouracil (5-FU)-loaded Poly(N-vinyl-2-pyrrolidone) (PVP) core for synergistic therapy of melanoma skin cancer. The yielded nanofibers exhibited an average diameter of 503 nm with high drug-encapsulating efficiency and good mechanical properties. Moreover, the burst release of 5-FU significantly inhibited melanoma skin cancer cells (B16F10 cells), and the sustained release of CS exhibited “remedying effects” on normal skin cells (L929 cells) after suffering adverse effects from 5-FU treatment. For the B16F10 cells, the early apoptosis cells increased from 0.8% to 62.2% after being treated with blended films loaded with 5-FU (2 wt%) for 24 h; for the L929 cells, the vital cells increased from 68.9% to 77.0%, and the early apoptosis of stage cells decreased from 12.3% to 10.9% after being treated with blended films with CS (8 wt%) for 24 h. In conclusion, the results introduced in this work can be a promising strategy for cancer treatment and possesses synergism potential to broaden an avenue for chemotherapeutic therapy with minimum adverse effects on normal cells.
KW - 5-Fluorouracil
KW - Chitosan
KW - Controlled release
KW - Core-shell nanofiber
KW - Skin cancer
UR - http://www.scopus.com/inward/record.url?scp=85068888727&partnerID=8YFLogxK
U2 - 10.1016/j.ejps.2019.105002
DO - 10.1016/j.ejps.2019.105002
M3 - Article
VL - 137
JO - European Journal of Pharmaceutical Sciences
T2 - European Journal of Pharmaceutical Sciences
JF - European Journal of Pharmaceutical Sciences
SN - 0928-0987
M1 - 105002
ER -