A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens

Luciana Terra, Paul Dyson, Matthew Hitchings, Liam Thomas, Alyaa Abdelhameed, Ibrahim M. Banat, Salvatore Gazze, Dusica Vujaklija, Paul Facey, Lewis Francis, Gerry Quinn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.
LanguageEnglish
JournalFrontiers in Microbiology
DOIs
Publication statusPublished - 16 Oct 2018

Fingerprint

Streptomyces
Anti-Bacterial Agents
Soil
Antiporters
Bacteria
Acinetobacter baumannii
Enterococcus faecium
Radon
Carbapenems
Gamma Rays
Methicillin-Resistant Staphylococcus aureus
Multigene Family
Microbial Drug Resistance
Growth
Cross Infection
Ireland
Computer Simulation
Genes
Software
Research Personnel

Keywords

  • alkaliphile
  • soil
  • antimicrobial
  • Streptomyces
  • ESKAPE pathogens
  • multi-resistant
  • ethnopharmacology

Cite this

Terra, L., Dyson, P., Hitchings, M., Thomas, L., Abdelhameed, A., Banat, I. M., ... Quinn, G. (2018). A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2018.02458
Terra, Luciana ; Dyson, Paul ; Hitchings, Matthew ; Thomas, Liam ; Abdelhameed, Alyaa ; Banat, Ibrahim M. ; Gazze, Salvatore ; Vujaklija, Dusica ; Facey, Paul ; Francis, Lewis ; Quinn, Gerry. / A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens. In: Frontiers in Microbiology. 2018.
@article{95ea956de32a437a86f140548e7ec9ed,
title = "A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens",
abstract = "In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57{\%} of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.",
keywords = "alkaliphile, soil, antimicrobial, Streptomyces, ESKAPE pathogens, multi-resistant, ethnopharmacology",
author = "Luciana Terra and Paul Dyson and Matthew Hitchings and Liam Thomas and Alyaa Abdelhameed and Banat, {Ibrahim M.} and Salvatore Gazze and Dusica Vujaklija and Paul Facey and Lewis Francis and Gerry Quinn",
year = "2018",
month = "10",
day = "16",
doi = "10.3389/fmicb.2018.02458",
language = "English",
journal = "Frontiers in Microbiology",
issn = "1664-302X",

}

Terra, L, Dyson, P, Hitchings, M, Thomas, L, Abdelhameed, A, Banat, IM, Gazze, S, Vujaklija, D, Facey, P, Francis, L & Quinn, G 2018, 'A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens', Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2018.02458

A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens. / Terra, Luciana; Dyson, Paul; Hitchings, Matthew; Thomas, Liam; Abdelhameed, Alyaa; Banat, Ibrahim M.; Gazze, Salvatore; Vujaklija, Dusica; Facey, Paul; Francis, Lewis; Quinn, Gerry.

In: Frontiers in Microbiology, 16.10.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens

AU - Terra, Luciana

AU - Dyson, Paul

AU - Hitchings, Matthew

AU - Thomas, Liam

AU - Abdelhameed, Alyaa

AU - Banat, Ibrahim M.

AU - Gazze, Salvatore

AU - Vujaklija, Dusica

AU - Facey, Paul

AU - Francis, Lewis

AU - Quinn, Gerry

PY - 2018/10/16

Y1 - 2018/10/16

N2 - In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.

AB - In an effort to stem the rising tide of multi-resistant bacteria, researchers have turned to niche environments in the hope of discovering new varieties of antibiotics. We investigated an ethnopharmacological (cure) from an alkaline/radon soil in the area of Boho, in the Fermanagh Scarplands (N. Ireland) for the presence of Streptomyces, a well-known producer of antibiotics. From this soil we isolated a novel (closest relative 57% of genome relatedness) Streptomyces sp. capable of growth at high alkaline pH (10.5) and tolerant of gamma radiation to 4 kGy. Genomic sequencing identified many alkaline tolerance (antiporter/multi-resistance) genes compared to S. coelicolor M145 (at 3:1), hence we designated the strain Streptomyces sp. myrophorea, isolate McG1, from the Greek, myro (fragrance) and phorea (porter/carrier). In vitro tests demonstrated the ability of the Streptomyces sp. myrophorea, isolate McG1 to inhibit the growth of many strains of ESKAPE pathogens; most notably carbapenem-resistant Acinetobacter baumannii (a critical pathogen on the WHO priority list of antibiotic-resistant bacteria), vancomycin-resistant Enterococcus faecium, and methicillin-resistant Staphylococcus aureus (both listed as high priority pathogens). Further in silico prediction of antimicrobial potential of Streptomyces sp. myrophorea, isolate McG1 by anti-SMASH and RAST software identified many secondary metabolite and toxicity resistance gene clusters (45 and 27, respectively) as well as many antibiotic resistance genes potentially related to antibiotic production. Follow-up in vitro tests show that the Streptomyces sp. myrophorea, isolate McG1 was resistant to 28 out of 36 clinical antibiotics. Although not a comprehensive analysis, we think that some of the Boho soils’ reputed curative properties may be linked to the ability of Streptomyces sp. myrophorea, isolate McG1 to inhibit ESKAPE pathogens. More importantly, further analysis may elucidate other key components that could alleviate the tide of multi-resistant nosocomial infections.

KW - alkaliphile

KW - soil

KW - antimicrobial

KW - Streptomyces

KW - ESKAPE pathogens

KW - multi-resistant

KW - ethnopharmacology

U2 - 10.3389/fmicb.2018.02458

DO - 10.3389/fmicb.2018.02458

M3 - Article

JO - Frontiers in Microbiology

T2 - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -