A NONISOTOPIC DNA HYBRIDIZATION ASSAY FOR THE IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS ISOLATED FROM FOODS

IG Wilson, K Gilmour, JE Cooper, AJ Bjourson, J Harvey

Research output: Contribution to journalArticle

Abstract

A digoxygenin-labelled total genomic DNA probe was used to identify Staphylococcus aureus. Isolation and identification of organisms was possible in less than 4 days. Identification alone could be completed in less than 2 days, compared with over 5 days for identification of isolates by multipoint inoculation. The probe showed excellent discrimination of S. aureus from other staphylococci and from a wide range of bacteria commonly associated with milk and meat. The effectiveness of this probe was tested against cultural isolation of staphylococci in milk using Baird-Parker agar followed by identification using multipoint inoculation and API Staph. The probe gave comparable results to the conventional methods and, for large sample numbers, offered lower cost and greater ease of use.
LanguageEnglish
Pages43-54
JournalInternational Journal of Food Microbiology
Volume22
Issue number1
Publication statusPublished - Apr 1994

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Staphylococcus
nucleic acid hybridization
Staphylococcus aureus
milk
DNA probes
assays
agar
meat
genomics
bacteria
organisms
sampling
methodology

Cite this

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title = "A NONISOTOPIC DNA HYBRIDIZATION ASSAY FOR THE IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS ISOLATED FROM FOODS",
abstract = "A digoxygenin-labelled total genomic DNA probe was used to identify Staphylococcus aureus. Isolation and identification of organisms was possible in less than 4 days. Identification alone could be completed in less than 2 days, compared with over 5 days for identification of isolates by multipoint inoculation. The probe showed excellent discrimination of S. aureus from other staphylococci and from a wide range of bacteria commonly associated with milk and meat. The effectiveness of this probe was tested against cultural isolation of staphylococci in milk using Baird-Parker agar followed by identification using multipoint inoculation and API Staph. The probe gave comparable results to the conventional methods and, for large sample numbers, offered lower cost and greater ease of use.",
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A NONISOTOPIC DNA HYBRIDIZATION ASSAY FOR THE IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS ISOLATED FROM FOODS. / Wilson, IG; Gilmour, K; Cooper, JE; Bjourson, AJ; Harvey, J.

In: International Journal of Food Microbiology, Vol. 22, No. 1, 04.1994, p. 43-54.

Research output: Contribution to journalArticle

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