A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae

MA Crockard, AJ Bjourson, JE Cooper

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h, Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.
LanguageEnglish
Pages825-828
JournalMolecular Plant - Microbe Interactions
Volume12
Issue number9
Publication statusPublished - Sep 1999

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Pseudomonas syringae
Trifolium repens
Rhizobium
peroxidase
pathogens
salicylic acid
spraying
reverse transcriptase polymerase chain reaction
seedlings
tissues

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Crockard, MA ; Bjourson, AJ ; Cooper, JE. / A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae. In: Molecular Plant - Microbe Interactions. 1999 ; Vol. 12, No. 9. pp. 825-828.
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abstract = "Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h, Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.",
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Crockard, MA, Bjourson, AJ & Cooper, JE 1999, 'A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae', Molecular Plant - Microbe Interactions, vol. 12, no. 9, pp. 825-828.

A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae. / Crockard, MA; Bjourson, AJ; Cooper, JE.

In: Molecular Plant - Microbe Interactions, Vol. 12, No. 9, 09.1999, p. 825-828.

Research output: Contribution to journal › Article

TY - JOUR

T1 - A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae

AU - Crockard, MA

AU - Bjourson, AJ

AU - Cooper, JE

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N2 - Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h, Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.

AB - Temporal reverse transcription-polymerase chain reaction (RT-PCR) expression analyses were performed on Trprx2, a new white clover peroxidase, with roots challenged with homologous rhizobia, heterologous rhizobia, and a pathogen, Pseudomonas syringae. Low levels of Trprx2 expression were evident in all rhizobial treatments but in P.syringae-treated clover background expression was dramatically reduced within 1 h and was undetectable in treatments inoculated for more than 3 h, Spraying 4 mM salicylic acid onto seedlings increased Trprx2 expression. These data suggest a defensive role for Trprx2 in white clover and indicate active defense suppression by the pathogen.

M3 - Article

VL - 12

SP - 825

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JO - Molecular Plant - Microbe Interactions

T2 - Molecular Plant - Microbe Interactions

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Crockard MA, Bjourson AJ, Cooper JE. A new peroxidase cDNA from white clover: Its characterization and expression in root tissue challenged with homologous rhizobia, heterologous rhizobia, or Pseudomonas syringae. Molecular Plant - Microbe Interactions. 1999 Sep;12(9):825-828.

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