The key criteria for assessing the success of bone tissue engineering is the quality and quantity of the mineralization within scaffolds. It has been demonstrated that stem cells and isolated primary bone cells can be induced to generate minerals in in vitro culture when using osteogenesis medium. The accumulation of calcium ion and inorganic phosphate from culture medium serves as nucleating agents for the formation of hydroxyapatite, which is the main inorganic component of bone. Bone nodule formation is one of the hallmarks of mineralization in such culture. In this project, we undertook a morphological study in which human bone marrow –derived mesenchymal stem cells (hMSCs) and osteoblast cell line have been cultured into cell aggregates under various culture conditions. We investigated the effect of the culture substrates on the capability of the formation of cell aggregates in terms of size and numbers, and the capability of the aggregates expansion and mineralization. It has been revealed that the nature of substrate affected the size and numbers of the cell aggregates. The number of bone nodules and the minerals generated from cell aggregates were higher than in monolayer cell culture.
|Title of host publication||A Morphological Study of Cell Aggregations on Mineralization|
|Publication status||Published (in print/issue) - 2011|