A comparison of three different micro-tomography systems for accurate determination of microvascular parameters - art. no. 70780E

P. D. Lee, R. C. Atwood, P. Rockett, M. A. Konerding, J. R. Jones, Christopher Mitchell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The investigation of micro-vessel dimensions in 3D is currently problematic due to their complex structures and fine scale. Quantification of vascular parameters is important in several fields of biomedicine; including embryogenesis, wound healing, diseases characterized by uncontrolled angiogenesis (e.g. tumor growth and metastasis) and the development of implantable bio-materials where a functional vascular supply is critical to their successful integration into host tissue. However, techniques that can resolve the micron-scaled features of these capillary beds, such as scanning electron and confocal microscopy, do not allow for total image reconstitution in 3 D in thick tissue samples [1]. The present study describes the use of an in vivo corrosion casting technique that provides a stable replica of the microvascular network and the subsequent evaluation of three different mu CT systems in order to accurately quantify vessel dimensions. Stable replicas of micro-vascular networks in neonatal mouse eyes were first created using in vivo vascular corrosion casting and then imaged using a unique, laboratory scale, mu CT unit. This system combines a LaB6 cathode with high-performance electron optics to obtain a high resolution x-ray source. Novel image analysis was then applied to the reconstructions to quantify the morphological parameters of the hyaloid vascular plexi in the developing eyes of postnatal day 2 (P2) wild-type mice. These results are compared to synchrotron scans, establishing vascular casting and x-ray mu CT as a valid laboratory scale experimental method for accurate 3D quantification of the microvasculature, with potential applications to a wide variety of fields in biological and medical research.
Original languageEnglish
Title of host publicationUnknown Host Publication
EditorsSR Stock
PublisherSPIE
PagesE780
Number of pages1000
Volume7078
DOIs
Publication statusPublished - 2008
EventDEVELOPMENTS IN X-RAY TOMOGRAPHY VI -
Duration: 1 Jan 2008 → …

Publication series

NamePROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)
PublisherSPIE digital library

Conference

ConferenceDEVELOPMENTS IN X-RAY TOMOGRAPHY VI
Period1/01/08 → …

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    Lee, P. D., Atwood, R. C., Rockett, P., Konerding, M. A., Jones, J. R., & Mitchell, C. (2008). A comparison of three different micro-tomography systems for accurate determination of microvascular parameters - art. no. 70780E. In SR. Stock (Ed.), Unknown Host Publication (Vol. 7078, pp. E780). (PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE)). SPIE. https://doi.org/10.1117/12.795558