A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80

Robert Leslie James Graham, S. Naomi O'Loughlin, Catherine E. Pollock, Nigel Ternan, D. Brent Weatherly, Philip J. Jackson, Rick L. Tarleton, Geoffrey McMullan

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To further our understanding of the biology of the thermophilic bacterium Geobacillus thermoleovorans T80, we now report the first proteomic analysis of the insoluble subproteome of this isolate. A combination of both shotgun and multidimensional methodologies were utilized, and a total of 8628 peptides was initially identified by automated MS/MS identification software. Curation of these peptides led to a final list of 184 positive protein identifications. The proteins from this insoluble subproteome were functionally classified, and physiochemical characterization was carried out. Of 15 hypothetical conserved proteins identified, we have assigned function to all but four. A total of 31 proteins were predicted to possess signal peptides. In silico investigation of these proteins allowed us to identify four of the five bacterial classes of signal peptide, namely, (i) twin-arginine translocation; (ii) Sec-type; (iii) lipoprotein, and (iv) ABC transport. In addition, a number of proteins were identified that are known to be involved in the transport of compatible solutes, known to be important in microbial stress responses.
LanguageEnglish
Pages2465-2473
JournalJournal of Proteome Research
Volume5
Issue number9
DOIs
Publication statusPublished - Sep 2006

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Bacillus thermoleovorans
thermophilic microorganisms
proteomics
proteins
signal peptide
peptides
thermophilic bacteria
lipoproteins
arginine
solutes
stress response
Biological Sciences

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Graham, Robert Leslie James ; O'Loughlin, S. Naomi ; Pollock, Catherine E. ; Ternan, Nigel ; Weatherly, D. Brent ; Jackson, Philip J. ; Tarleton, Rick L. ; McMullan, Geoffrey. / A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 9. pp. 2465-2473.
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A combined shotgun and multidimensional proteomic analysis of the insoluble subproteome of the obligate thermophile, Geobacillus thermoleovorans T80. / Graham, Robert Leslie James; O'Loughlin, S. Naomi; Pollock, Catherine E.; Ternan, Nigel; Weatherly, D. Brent; Jackson, Philip J.; Tarleton, Rick L.; McMullan, Geoffrey.

In: Journal of Proteome Research, Vol. 5, No. 9, 09.2006, p. 2465-2473.

Research output: Contribution to journalArticle

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