5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels

Rachelle E Irwin, Avinash Thakur, Karla O'Neill, CP Walsh

Research output: Contribution to conferenceAbstract

Abstract

We recently identified a class of neuronal gene inheriting high levels of intragenic methylation from the mother and maintaining this through later development. We show here that these genes are implicated in basic neuronal functions such as post-synaptic signalling, rather than neuronal development and inherit high levels of 5mC, but not 5hmC, from the mother. 5mC is distributed across the gene body and appears to facilitate transcription, as transcription is reduced in DNA methyltransferase I (Dnmt1) knockout embryonic stem cells as well as in fibroblasts treated with a methyltransferase inhibitor. However in adult brain, transcription is more closely associated with a gain in 5hmC, which occurs without a measurable loss of 5mC. These findings add to growing evidence that there may be a role for 5mC in promoting transcription as well as its classical role in gene silencing. Further interrogation of the mechanisms behind the persistence of gametic marks could potentially lead to insights into neurological disorders.
LanguageEnglish
Pages212
Number of pages1
Publication statusPublished - 2015
Event18th Meeting of the Irish Society of Human Genetics - Dublin City University
Duration: 3 Sep 2015 → …

Conference

Conference18th Meeting of the Irish Society of Human Genetics
Period3/09/15 → …

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Methyltransferases
Genes
Gene Silencing
Embryonic Stem Cells
Nervous System Diseases
Methylation
Fibroblasts
DNA
Brain

Cite this

Irwin, R. E., Thakur, A., O'Neill, K., & Walsh, CP. (2015). 5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels. 212. Abstract from 18th Meeting of the Irish Society of Human Genetics, .
Irwin, Rachelle E ; Thakur, Avinash ; O'Neill, Karla ; Walsh, CP. / 5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels. Abstract from 18th Meeting of the Irish Society of Human Genetics, .1 p.
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Irwin, RE, Thakur, A, O'Neill, K & Walsh, CP 2015, '5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels' 18th Meeting of the Irish Society of Human Genetics, 3/09/15, pp. 212.

5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels. / Irwin, Rachelle E; Thakur, Avinash; O'Neill, Karla; Walsh, CP.

2015. 212 Abstract from 18th Meeting of the Irish Society of Human Genetics, .

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - 5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels

AU - Irwin, Rachelle E

AU - Thakur, Avinash

AU - O'Neill, Karla

AU - Walsh, CP

PY - 2015

Y1 - 2015

N2 - We recently identified a class of neuronal gene inheriting high levels of intragenic methylation from the mother and maintaining this through later development. We show here that these genes are implicated in basic neuronal functions such as post-synaptic signalling, rather than neuronal development and inherit high levels of 5mC, but not 5hmC, from the mother. 5mC is distributed across the gene body and appears to facilitate transcription, as transcription is reduced in DNA methyltransferase I (Dnmt1) knockout embryonic stem cells as well as in fibroblasts treated with a methyltransferase inhibitor. However in adult brain, transcription is more closely associated with a gain in 5hmC, which occurs without a measurable loss of 5mC. These findings add to growing evidence that there may be a role for 5mC in promoting transcription as well as its classical role in gene silencing. Further interrogation of the mechanisms behind the persistence of gametic marks could potentially lead to insights into neurological disorders.

AB - We recently identified a class of neuronal gene inheriting high levels of intragenic methylation from the mother and maintaining this through later development. We show here that these genes are implicated in basic neuronal functions such as post-synaptic signalling, rather than neuronal development and inherit high levels of 5mC, but not 5hmC, from the mother. 5mC is distributed across the gene body and appears to facilitate transcription, as transcription is reduced in DNA methyltransferase I (Dnmt1) knockout embryonic stem cells as well as in fibroblasts treated with a methyltransferase inhibitor. However in adult brain, transcription is more closely associated with a gain in 5hmC, which occurs without a measurable loss of 5mC. These findings add to growing evidence that there may be a role for 5mC in promoting transcription as well as its classical role in gene silencing. Further interrogation of the mechanisms behind the persistence of gametic marks could potentially lead to insights into neurological disorders.

M3 - Abstract

SP - 212

ER -

Irwin RE, Thakur A, O'Neill K, Walsh CP. 5-Hydroxymethylation marks a class of neuronal gene regulated by intragenic methylcytosine levels. 2015. Abstract from 18th Meeting of the Irish Society of Human Genetics, .